02352nas a2200217 4500008004100000245014700041210006900188260001800257300001200275490000600287520163200293653001601925653003201941653001701973653001701990653002902007100002202036700001602058700002502074856003502099 2017 eng d00aAntioxidant Activity and Lipoxygenase Enzyme Inhibitory Assay with Total Flavonoids Content from Garcinia hombroniana Pierre Stem Bark Extract0 aAntioxidant Activity and Lipoxygenase Enzyme Inhibitory Assay wi cFebruary 2017 a276-2790 v93 a

Introduction: Garcinia has been known as a rich source of xanthones, flavonoids, and phenols. The aim of this research is to obtain data of antioxidant activity and to observe potential inhibition of lipoxygenase activity that most active from methanolic, ethyl acetate and n-hexane extracts with total flavonoids content from most active extracts from the bark of Garcinia hombroniana Pierre. Methods: The antioxidant activity was measured using the ferric reducing antioxidant power (FRAP), the anti-inflammatory assay was measured using inhibition of lipoxygenase activity test, qualitative analysis of flavonoids using thin layer chromatography, and total flavonoids content was measured using AlCl3 colorimetric method. Results: The results showed that the ethyl acetate extract from G. hombroniana Pierre stem bark as the most active extract for antioxidant and lipoxygenase inhibition activity with EC50 and IC50 value consecutively 15.34 μg /ml; 0.26 μg /ml. Total flavonoids content of ethyl acetate is 7.430 mg QE/g extract. The results of this study showed bark extract Garcinia hombroniana Pierre has antioxidant activity and potent to inhibit lipoxygenase activity. Conclusion: Based on the research for methanolic, ethyl acetate and n-hexane extract, it can be concluded that the ethyl acetate extract of G. hombroniana Pierre as the most active extract for antioxidant and lipoxygenase inhibition activity.

10aAntioxidant10aGarcinia hombroniana Pierre10aInflammation10aLipoxygenase10aTotal flavonoids content1 aListiyani, Amanda1 aElya, Berna1 aPuspitasari, Nuraini uhttp://phcogj.com/fulltext/314