02308nas a2200217 4500008004100000245013100041210006900172260001800241300001200259490000600271520162300277653001601900653002201916653000901938653002801947653001701975100002201992700001602014700002502030856003502055 2017 eng d00aAntioxidant Activity and Lipoxygenase Inhibition Test with Total Flavonoid Content from Garcinia kydia Roxburgh Leaves Extract0 aAntioxidant Activity and Lipoxygenase Inhibition Test with Total cFebruary 2017 a280-2840 v93 a

Introduction: Antioxidant is one of the therapeutic strategies to overcome oxidative stress and inhibit synthesis of inflammatory mediators through lipoxygenase pathway. Garcinia is the largest of Clusiaceae family which has been proven to provide antioxidant and anti-inflammatory activity. Garcinia kydia Roxburgh is one of the plants of this genus which is known to have antioxidant activity but lipoxygenase inhibition activity from this plant was unknown. Methods: This study aimed to test antioxidant activity of the methanol, ethyl acetate and n-hexane extract from Garcinia kydia Roxburgh leaves by FRAP (Ferric Reducing Antioxidant Power) method, anti-inflammatory activity was tested by inhibiting lipoxygenase and total flavonoid content by colorimetric methods AlCl3. Results: The results showed an antioxidant activity of methanol extract, ethyl acetate and n-hexane leaves of Garcinia kydia Roxburgh have EC50 value, respectively 18,448; 12,389 and 31,260 μg/mL, and the lipoxygenase inhibition activity have IC50 value, respectively 0,556; 0,212 and 3,575 μg/mL. Ethyl acetate extract of Garcinia kydia Roxburgh leaves was the most active extract in this study which has total flavonoid content, 30,650 mgQE/ gram extract. Conclusion: The conclusion, Garcinia kydia Roxburgh has antioxidant and lipoxygenase inhibition activity, with ethyl acetate extract as the most active extract which contains total flavonoids.

10aAntioxidant10aFlavonoid content10aFRAP10aGarcinia kydia Roxburgh10aLipoxygenase1 aPutri, Nur, Laily1 aElya, Berna1 aPuspitasari, Nuraini uhttp://phcogj.com/fulltext/315