01883nas a2200229 4500008004100000245011200041210006900153260001800222300001200240490000600252520118800258653001801446653001401464653003101478653001301509100003001522700000501552700000501557700002501562700003101587856003501618 2017 eng d00aACE Inhibitory Activity, Total Phenolic and Flavonoid Content of Pereskia saccharose Griseb. Leaves Extract0 aACE Inhibitory Activity Total Phenolic and Flavonoid Content of  cFebruary 2017 a285-2870 v93 a

Introduction: Angiotensin-converting enzyme inhibitors (ACEi) are drugs that can control hypertension. Pereskia saccharose Griseb. leaves have been used traditionally as antihypertensive. Objective: The objective of this study was to determine the antihypertensive activity through inhibition of ACE activity, the total phenolic content and total flavonoid content of the ethanolic extract of Pereskia saccharose Griseb. leaves and its fractions. Methods: Extraction was done by maceration with 80% ethanol and fractionation performed by liquid-liquid partition. Results: In vitro ACE inhibitory activity assay of the ethanolic extract using ACE Kit-WST Dojindo had IC50 value of 3.448 μg/mL and ethyl acetate fraction had IC50 value of 1.714 x 10-3 μg/mL. Ethyl acetate contained the highest amounts of both TPC (72.991 ± 0.932 mg GAE/g sample) and TFC (61.337 ± 1.612 mg QE/g sample). Conclusion: The results suggest that Pereskia saccharose Griseb. possess ACE inhibitory activity.

10aACE inhibitor10aFlavonoid10aPereskia saccharose Griseb10aphenolic1 aLusiyanti, Sarlina, Jihan1 a1 a1 aPuspitasari, Nuraini1 aMahayasih, Putu, Gita Maya uhttp://phcogj.com/fulltext/316