02755nas a2200301 4500008004100000245015700041210006900198260001300267300001200280490000600292520169800298653002501996653002102021653002102042653001902063100003502082700003202117700002102149700003002170700003102200700003602231700002902267700002702296700003102323700003002354700003102384856003802415 2017 eng d00aEffects of Rainfall on the Antimicrobial Activity and Secondary Metabolites Contents of Leaves and Fruits of Anadenanthera colubrina from Caatinga Area.0 aEffects of Rainfall on the Antimicrobial Activity and Secondary  cMay 2017 a435-4400 v93 a

Background: Anadenanthera colubrina (Vell.) Brenan var. cebil (Griseb) is a plant widely used for medicinal proposes in Brazilian Northeast. Objective: This study aimed to analyze the influence of rainfall indexes (RI) in antimicrobial activity and phytochemical constituents of extracts from leaves and fruits of A. colubrina. Material and Methods: Samples were collected in Catimbau National Park (Buíque, Pernambuco, Brazil) at September 2010 (RI: 75 mm) and January (RI: 65 mm), April (RI: 162 mm) and June 2011 (RI: 73 mm). The extracts were prepared by Soxhlet extraction using cyclohexane, chloroform, ethyl acetate and methanol. The antimicrobial activity was determined by MIC and MBC values. Results: All extracts showed antimicrobial activity, but ethyl acetate extracts (from all periods) were more active. Strong correlations were found between the RI and the average MIC of MLE (ρ: -0.99), EALE (ρ: -0.81), CHFE (ρ: -0.81), EAFE (ρ: -0.80); while moderate and weak correlations were found for other extracts. Through a HPLC analysis was possible to reveal that the samples collected from dry periods had more chemical diversity (as they presented more peaks). Gallic acid and quercetin (and derivative compounds) were identified. The levels of quercetin were enhanced in extracts from dry months. Conclusion: Our results showed that the rainfall has a positive effect on the antimicrobial activity of leaves and fruits of A. colubrina, however these extracts showed more chemical diversity during dry months.

10aAntimicrobial agents10aMedicinal plants10aNatural products10aSemi arid area1 ade Araújo, Daniel, Rodrigo Ca1 ada Silva, Luís, Cláudio N1 aHarand, Wolfgang1 aFernandes, Júlia, Morais1 aSoares, Thaciane, da Cunha1 aLangassner, Silvana, Maria Zuco1 aGiordani, Raquel, Brandt1 aXimenes, Rafael, Matos1 ada Silva, Alexandre, Gomes1 ada Silva, Márcia, Vanusa1 aCorreia, Maria, Tereza dos u/files/PJ-9-4/10.5530pj.2017.4.73