02833nas a2200265 4500008004100000245012900041210006900170260001400239300001200253490000600265520198100271653002602252653001702278653001602295653002502311653001602336100002802352700002202380700003002402700001902432700001602451700002902467700002102496856005002517 2017 eng d00aGC-MS analysis of Tasmannia lanceolata Extracts which Inhibit the Growth of the Pathogenic Bacterium Clostridium perfringens0 aGCMS analysis of Tasmannia lanceolata Extracts which Inhibit the cJuly 2017 a626-6370 v93 a

Introduction: Clostridium perfringens is the etiological agent of clostridial myonecrosis and enteritis necroticans. Infections result in exotoxin production, tissue necrosis and unless promptly treated, often result in death. Methods: Tasmannia lanceolata extracts were investigated for C. perfringens growth inhibitory activity by disc diffusion analysis and MIC determination. Toxicity was evaluated by Artemia nauplii bioassay and the most potent extracts were phytochemically evaluated by GC-MS headspace analysis. Results: All T. lanceolata berry and leaf extracts displayed potent C. perfringens growth inhibition. The berry extracts were more potent growth inhibitors than the corresponding leaf extracts, although the leaf extracts were also potent growth inhibitors. The berry aqueous, methanolic and ethyl acetate extracts were particularly potent growth inhibitors, with MIC values of 654, 65 and 329 μg/mL respectively. T. lanceolata leaf also displayed good efficacy, with an MIC of 839, 1255 and 625 μg/mL for the aqueous, methanolic and ethyl acetate extracts respectively. All extracts were nontoxic in the Artemia franciscana bioassay, with LC50 values substantially > 1000 μg/mL. Non-biased GC-MS analysis of the aqueous, methanolic and ethyl acetate berry extracts revealed the presence of high relative levels of a diversity of terpenoids. Conclusions: The lack of toxicity of the T. lanceolata extracts and their potent growth inhibitory bioactivity against C. perfringens indicates their potential as medicinal agents in the treatment and prevention of clostridial myonecrosis and enteritis necroticans. GC-MS metabolomic profiling studies indicate that these extracts contained a diversity of terpenoids, with monoterpenoids being particularly abundant.

10aEnteritis necroticans10aGas gangrene10aMyonecrosis10aTasmannia Lanceolata10aWinteraceae1 aWright, Mitchell, Henry1 aLee, Cameron, Jay1 aArnold, Megan, Sarah Jean1 aShalom, Joseph1 aWhite, Alan1 aGreene, Anthony, Carlson1 aCock, Ian, Edwin u/files/pj-9-5/10.5530pj.2017.5.100/index.html