02574nas a2200217 4500008004100000245022300041210006900264260001900333300001200352490000600364520177500370653002402145653001002169653001402179653002102193653002202214100002902236700001902265700003702284856003502321 2017 eng d00aInfluence of Extraction Parameters on the Yield, Phytochemical, TLC-Densitometric Quantification of Quercetin, and LC-MS Profile, and how to Standardize Different Batches for Long Term from Ageratum conyoides L. Leaves0 aInfluence of Extraction Parameters on the Yield Phytochemical TL cSeptember 2017 a767-7740 v93 a

Introduction: Ageratum conyzoides Linn is one of the Indonesian herbs are used as folk medicine. There are many studies to the therapeutic activity. However, the study of the influence in extraction parameters and how to standardize different batches of plant is still limited. Objective: The present study was designed to investigate the influence of extraction parameters on the variables matter, yield, phytochemical profile, and total quercetin from A. conyzoides L. leaves extract. The study also evaluates Liquid Chromatography-Mass Spectroscopy (LC-MS) and TLC fingerprint profiles of the extract to determine the proper quality of plant material of A. conyzoides L. leaves. Methods: Different temperatures (from 0°C to 60°C), solvent types (water and 70% ethanol (%v/v), and extraction time (192 h VS 2 h) were used to investigate the influence of extraction parameters. The profiles of extract were performed by TLC and LC-MS method. Results: The extract yield was influenced by temperature, solvent types, and time extraction. Our results suggest that the digestion technique at 60°C with 70% ethanol for 2h was recommended for the extraction method to obtained high yield and quercetin content as bioactive compound in A. conyzoides L. leaves extract. The range of quercetin content (40–52 ppm) combined with TLC and LC-MS profile can be used to determine the proper quality of plant material. Conclusion: All parameter of extraction have an affect on the yield extract and total quercetin on the final extract. Thus, there is a need an optimized method to obtain the suitable method.

10aAgeratum conyzoides10aLc-Ms10aQuercetin10aStandardization.10aTlc-Densitometric1 aTambunan, Anita, Paulina1 aBahtiar, Anton1 aTjandrawinata, Raymond, Rubianto uhttp://fulltxt.org/article/174