01996nas a2200217 4500008004100000245009800041210006900139260001900208300001200227490000600239520132900245653001801574653004001592653001501632653001501647653002101662100002101683700001701704700002201721856003501743 2017 eng d00aIn vitro Study of Xanthine Oxidase Inhibitory of Gambir (Uncaria gambir) Hunter Roxb Extracts0 aIn vitro Study of Xanthine Oxidase Inhibitory of Gambir Uncaria  cSeptember 2017 a862-8650 v93 a

Introduction: Hyperuricemia was a metabolic disorder characterized by high levels of uric acid due to the action of the enzyme xanthine oxidase (XO). Some natural substances with antioxidant activity proved capable of inhibiting the activity of the enzyme XO. Pharmacological benefit of polyphenol compounds had been proved. Gambir (Uncaria gambir) Hunter Roxb, a native plant, had been proved antioxidant activity, so that it had potential to be developed as an inhibitor of the XO. This study aimed to evaluate the activity of Gambir as XO inhibitor. Methods: These extracts of Gambir were preparation from Gambir and Gambir leaf using ethanol 50% and ethanol 96%, respectively. The polyphenol content and Xanthine oxidase inhibitory activity was evaluated by spectrophotometry, meanwhile analysis of (+)catechin was determined by high pressure liquid chromatography (HPLC). Results: Screening XO inhibitory activity in vitro showed that ethanolic 96% extract of Gambir leaf showed the highest activity, i.e. 50% relative to standard allopurinol at the final concentration of 100 ppm. Conclusion: There was no positive correlation between XO inhibitory activity and polyphenol or (+)catechin content.

10a(+) Catechin.10aGambir (Uncaria gambir) Hunter Roxb10aInhibitory10aPolyphenol10aXanthine Oxidase1 aRismana, Eriawan1 aNingsih, Sri1 aFachrudin, Fachry uhttp://fulltxt.org/article/188