02953nas a2200265 4500008004100000245013700041210006900178260001900247300001200266490000600278520206100284653002902345653002202374653002102396653001702417653003302434653002602467653002502493100003002518700002502548700003102573700002902604700001902633856003502652 2017 eng d00aExtracellular L-Asparaginase from Streptomyces labedae VSM-6: Isolation, Production and Optimization of Culture Conditions Using RSM0 aExtracellular LAsparaginase from Streptomyces labedae VSM6 Isola cSeptember 2017 a932-9410 v93 a

Objective: The present study was intended to isolate actinomycetes VSM-6 from deep sea sediment samples of Bay of Bengal that is potent to produce L - asparaginase. Materials and Methods: The identification of the isolate was executed by polyphasic taxonomy. Optimization was carried out one factor at a time (O-F-A-T) for the production of the L - asparaginase. RSM was pledged to optimize the L - asparaginase production by S.labedae VSM-6. Central composite design was applied to study the influence of the variables and their interactive effects on the production of L - asparaginase. Unstructured Kinetic modelling for L - asparaginase production was adopted using Leudeking-Piret (LILP) and Logistic Incorporated Modified Leudeking-Piret (LIMLP) models. Results: Optimization using One-Factor-At-A-time registered a turnout of 8.92 IU/ml of L - asparaginase production. But results obtained from the statistical design are in agreement with the experimental results. The model followed the second order polynomial equation and the model adequacy was determined by the P value (<0.0001), Coefficient determination (R2) with a value of 0.9942 and the adjusted R2 = 0.9087 which determines that the model was significant. The experimental values are in compliance with the model anticipated values and catalogued an escalation in yield of L - asparaginase (10.17 IU/ml) by RSM. Unstructured Kinetic modelling for L - asparaginase production adopting Leudeking-Piret (LILP) and Logistic Incorporated Modified Leudeking-Piret (LIMLP) models showed L - asparaginase production of (10.17 IU/ml), closer to model anticipated value (10.23 IU/ml) so unstructured models provided a better approximation for L - asparaginase production by S.labedae VSM-6. Conclusion: From our study we have reported for the first time the production of L - asparaginase from S.labedae VSM-6 using central composite design and kinetic modelling.

10aCentral Composite Design10aKinetic Modelling10aL - asparaginase10aOptimization10aResponse Surface Methodology10aStatistical Analysis.10aStreptomyces labedae1 aMangamuri, Ushakiranamayi1 aVijayalakshmi, Muvva1 aGanduri, Venkat, Siva Rama1 aRajulapati, Satish, Babu1 aPoda, Sudhakar uhttp://fulltxt.org/article/199