02144nas a2200217 4500008004100000245013800041210006900179260001800248300001400266490000600280520147600286653001601762653000901778653001601787653001001803653002101813100002001834700002001854700001701874856003501891 2017 eng d00aPharmacognostic Evaluation and Determination of Secondary Plant Metabolites by HPTLC and its Antioxidant Activity in Myrica esculenta0 aPharmacognostic Evaluation and Determination of Secondary Plant  cNovember 2017 as103-s1060 v93 a

Introduction: Myrica esculenta Buch.-Ham. (Myricaceae) is commonly known as Box Berry, Kaiphal, and Katphala in Ayurveda. As per the Ayurvedic literature the palnt is used for variety of diseases and disorders. Method: The present study deals with the pharmacognostical standardization, HPTLC analysis and antioxidant activity of methanolict extracts of the leaves of M. esculenta. The plant showed high phenolic and flavonoid content. In-vitro antioxidant study of dried leaves of Myrica esculanta was performed using methanolic extract. Results: Antioxidant activity of M. esculenta methanolic extract showed the least IC50 value of 60 ± 1.15 μg/ml. Standard ascorbic acid showed an IC50 value of 2.03 ± 0.06 μg/ml. The calibration curve of Gallic acid showed r2 of 0.949 and Rf of gallic acid was found to be 0.44 ± 0.006. Quantification of gallic acid in the samples of leaves of M. esculenta has been performed and the gallic acid was found to be 0.056%. Conclusion: The presence of gallic acid has not yet been reported and quantified in this species which may be utilized for the proper standardization of the drug. The present study showed new natural antioxidant that can replace the synthetic ones to be used in foods and cosmetics.

10aAntioxidant10aDPPH10aGallic acid10aHPTLC10aMyrica Esculenta1 aKumar, Prashant1 aGupta, Abhishek1 aSingh, Anita uhttp://fulltxt.org/article/390