02389nas a2200229 4500008004100000245013700041210006900178260001800247300001100265490000700276520165400283653001801937653001601955653001901971653003301990653001902023100002202042700001902064700001802083700002302101856003502124 2018 eng d00aPhytochemical Analysis of Nigella sativa L. Utilizing GC-MS Exploring its Antimicrobial Effects against Multidrug-Resistant Bacteria0 aPhytochemical Analysis of Nigella sativa L Utilizing GCMS Explor cDecember 2017 a99-1050 v103 a

Introduction: The alarming increase in bacterial strains resistant to existing antimicrobial agents has demanded alternative strategies. Medicinal plants are now considered as an alternative treatment because of their secure choice in several diseases. Among them, Nigella sativa is a promising traditional herb having rich medical background. The aim of the study is to perform phytochemical analysis of Nigella sativa L. Utilizing Gas chromatographic–mass spectrometric (GC/MS) exploring its antioxidant and antibacterial activity against multidrug resistant (MDR) gram-positive and gram-negative bacteria. Methods: Total phenolic, tannin, and flavonoid content of N. sativa seed extracts and its commercially available oil were determined. Their radical scavenging activity using DPPH was also tested. The antibacterial activity of N. sativa seed extracts and its oil against MDR gram-positive and gram-negative bacterial strains was studied using disc diffusion test and the biofilm formation assay. GC-MS studies were also performed. Results: Among the different preparations used, N. sativa oil showed the highest antioxidant and antibacterial activity against highly resistant gram-positive bacteria with the greatest suppression of biofilm formation, which was attributed to its high bioactive contents. Conclusion: This study indicates that N. sativa extracts and its oils can be used as natural antibacterial agents to treat infections caused by multidrug resistant bacteria.

10aAntibacterial10aAntioxidant10aGC-MS analysis10aMultidrug resistant bacteria10aNigella sativa1 aSaleh, Fatima, A.1 aEl-Darra, Nada1 aRaafat, Karim1 aGhazzawi, Iman, El uhttp://fulltxt.org/article/404