02108nas a2200217 4500008004100000245007300041210006900114260001700183300001200200490000600212520142000218653001001638653002401648653004201672653003901714653001901753100002701772700002801799700002901827856003401856 2015 eng d00aRapid and high yield Extraction method for Saponins from Safed musli0 aRapid and high yield Extraction method for Saponins from Safed m cJul-Aug 2015 a210-2140 v73 a

Objectives: We aimed to develop, compare and optimise rapid and high yield extraction method for saponins of Safed musli using conventional extraction techniques and as well as modern microwave assisted solvent extraction method. Materials and methods: Roots of Safed musli (Chlorophytum borivilianum) are extracted by maceration, soxhlet, sonication and microwave methods. Extract further fractionated to obtain total saponins. Microwave assisted solvent extraction (MASE) method is optimised using Taguchi L9 orthogonal array design. Total saponins are estimated by High Performance Thin Layer chromatography (HPTLC) from all extracts obtained by different methods. Results: Factors namely temperature, irradiation time, irradiation power and powder size which potentially affects extraction efficiency are considered while optimizing MASE by statistical orthogonal array design procedure and saponins are quantified using HPTLC. Under developed optimum conditions, MASE showed significantly higher yield (5.11%) and drastic reduction in extraction time (4 min) than conventional extraction methods. Conclusion: Saponins of Safed musli shown highest yield in MASE and then maceration, soxhlet and sonication followed. The developed and optimised method of saponin extraction by MASE can have huge industrial applications after scale up.

10aHPTLC10aMaceration Saponins10aMicrowave assisted solvent extraction10aOrthogonal test L9 (34) Sonication10aTaguchi Design1 aDeore, Sharada, Laxman1 aBaviskar, Bhushan, Arun1 aRangari, Ashwini, Suresh uhttps://phcogj.com/article/50