02610nas a2200217 4500008004100000245011100041210006900152260001700221300001200238490000700250520189100257653001902148653003402167653003602201653001202237653004102249100002902290700002002319700001802339856003502357 2018 eng d00aBioassay Guided Fractionation and in vitro Anti-plasmodial Activity of Ficus deltoidea and Ficus benjamina0 aBioassay Guided Fractionation and in vitro Antiplasmodial Activi cJanuary 2018 a235-2400 v103 a

Background: Malaria is infectious vector born disease affecting 212 million people belonging to 97 countries globally in the year 2016. Although this number has reduced progressively from last one decade but recent failure of currently available antimalarial drug therapy has accentuated the urgent need to explore different novel approaches in Anti-plasmodial drug discovery. Objective: The aim of the present study was to evaluate the Anti-plasmodial activity of traditional medicinal plants Ficus deltoidea and Ficus benjamina. Materials and Methods: Crude petroleum ether and hydro alcoholic extract of both the plant species were evaluated for Anti-plasmodial activity by schizont maturation inhibition assay using 3D7 plasmodium strains. Results: It was observed that petroleum ether extract of F. benjamina leaves showed most promising inhibitory effect on the growth of schizonts with IC50 14.5 μg/ml. Bio-assay guided fractionation of petroleum ether extract of F. benjamina led to the hexane and chloroform fraction with high Anti-plasmodial activity (IC50 4.0 μg/ml and IC50 7.8 μg/ml respectively). Further, phytochemical investigation of F. benjamina indicated the presence of various valuable phytochemicals belonging to class of steroids, terpenoids and phytosterols. Conclusion: This study has revealed the Anti-plasmodial activity of F. deltoidea and F. benjamina for the first time. Significant Anti-plasmodial activity and preliminary phytochemical studies of F. benjamina indicates its rich chemical diversity which make this plant a good candidate for isolating new molecule that could serve as new lead in Anti-plasmodial drug discovery. 

10aAntiplasmodial10aBioassay guided fractionation10aFicus deltoidea Ficus benjamina10aMalaria10aSchizont maturation inhibition assay1 aMukhtar, Hayat, Mohammad1 aSingh, Amandeep1 aKaur, Hardeep uhttp://fulltxt.org/article/471