02627nas a2200253 4500008004100000245011000041210006900151260001200220300001200232490000600244520183100250653000902081653002402090653001002114653000902124653002202133653003702155100004002192700002702232700002902259700003102288700002002319856003402339 2015 eng d00aAntioxidant and DNA Damage Protective Effects of Asparagus racemosus in Human Colon and Mice Muscle Cells0 aAntioxidant and DNA Damage Protective Effects of Asparagus racem c01/2015 a182-1900 v73 a

Introduction: The present study was designed to investigate the in vitro antioxidant and macromolecule damage protective effects of Asparagus racemosus water (AWE) and methanolic (AME) fractions of roots. Methods: The in vitro antioxidant activity of AWE/AME was estimated by free radical scavenging assays. The DNA damage of HT29 and C2C12 cells was analyzed by comet assay. The plasmid DNA damage and protein oxidation were carried out by agarose gel electrophoresis and SDS-PAGE analysis respectively, where as lipid peroxidation was performed by TBARS assay. Results: Both the extracts showed scavenging activity with IC50 values of 417.4 ± 19.5 / 298 ± 13.5, 381 ± 18.2 / 235 ± 11.9, 54.8 ± 2.95 / 31.6 ± 1.52, 28.9 ± 1.73 / 19.7 ± 1.55 μg/mL for DPPH, metal chelating, ABTS and Nitric oxide scavenging activities respectively. Similarly the methanolic extract showed more potent reducing power and total antioxidant activities over water fraction. The AME showed 56.8% and 41.2% protection against H2O2 (Hydrogen peroxide) induced DNA damage of HT29 human colon cells and C2C12 murine myoblasts. The extract also showed protection against H2O2 induced plasmid DNA damge, AAPH induced protein oxidation of bovine serum albumin (BSA) and lipid peroxidation of rat hepatic tissue. Conclusion: Over all this study showed remarkable antioxidant and macromolecule damage protective effects of A. racemosus. The observed biological properties may be attributed to the high content phenols and flavonoids in the methanolic extract A. racemosus over water extract.

10aAAPH10aAsparagus racemosus10aC2C1210aHT2910aProtein oxidation10aSingle cell gel electrophoresis.1 aJayashree, Girindrababu, Venkattapp1 aRachitha, Puttasiddiah1 aKrupashree, Krishnaswamy1 aKumar, Kandikattu, Hemanth1 aKhanum, Farhath uhttps://phcogj.com/article/53