02221nas a2200241 4500008004100000245018900041210006900230260001500299300001200314490000700326520143300333653001401766653000901780653001501789653001101804653002001815653002001835653001401855100002301869700002601892700002601918856003501944 2018 eng d00aDetection and Quantification of Major Phytochemical Markers for Standardization of Talinum Portulacifolium, Gomphrena Serrata, Alternanthera Sessilis and Euphorbia Heterophylla by HPLC0 aDetection and Quantification of Major Phytochemical Markers for  cMarch 2018 a439-4460 v103 a

Background: High-performance liquid chromatography is one of the major analytical techniques used in the quality control of phytochemicals. Objective: This research article presents the development of HPLC method to detect and quantify the major marker components, kaempferol, and quercetin from four plant species. Materials and Methods: HPLC method was developed for the qualitative and quantitative analysis of plant extracts by using orthophosphoric acid and methanol (95:5) at 370 nm for kaempferol, methanol and orthophosphoric acid (60:40) at 262nm for quercetin. Results: Kaempferol was detected from the hydro alcoholic extracts of Talinum portulacifolium leaves (RT 13.720, concentration 1.08 mg/ml) and flowers of Gomphrena serrata (RT 13.758, concentration 2.13mg/ml). Kaempferol was reported for the first time from Gomphrena serrata. Quercetin was separated and identified from the hydro alcoholic extracts Alternanthera sessilis stems (RT 6.503, concentration 0.01mg/ml). The hydroalcoholic extract of Euphorbia heterophylla stems (RT 6.588, concentration 0.01mg/ml) was also evaluated for the presence of quercetin. Conclusion: The method developed is very useful tool for qualifying and quantifying the plant specimens as well as their extracts.

10aExtracts.10aHPLC10aKaempferol10aMarker10aPlant specimens10aQuality control10aQuercetin1 aVani, Mamillapalli1 aRahaman, Shaik, Abdul1 aRani, Avula, Prameela uhttp://fulltxt.org/article/505