02503nas a2200217 4500008004100000245015200041210006900193260001300262300001200275490000700287520181200294653001702106653001602123653001602139653001302155653002402168100001702192700002102209700002002230856003502250 2018 eng d00aIn-vitro Evaluation of Antioxidant Activity and Anti-collagenase Activity of Thalassia hempricii as a Potent Ingredients for Anti-Wrinkle Cosmetics0 aInvitro Evaluation of Antioxidant Activity and Anticollagenase A cMay 2018 a778-7820 v103 a

Background: Thalassia hemprichii have reported containing antioxidant effects. However, information on other biological activities relating to the anti-wrinkle properties is limited. The free radical scavenging activity and enzyme inhibitory activity of the plant extracts investigated that they can help restore skin elasticity and thereby slow the wrinkling process. Objective: To evaluate in-vitro antioxidant and anti-collagenase activity of T. hemprichii as a potent ingredient for anti-wrinkle cosmetic. Methods: T. hemprichii was extracted with ethanol 100% (E1) 75% (E2) and ethanol 50% (E3) using maceration extraction method for 24 h, thrice. The extract was examined for total phenolic content, antioxidant activity compared with vitamin C, and the best extract was tested for the inhibitory activity of collagenase. Results: The total phenolic content of T. hempricii extract was 0,525 ± 0,016 mg GAE/100 g extract (E1) 0,538 ± 0,006 mg GAE/100 g extract (E2) and 0,558 ± 0,090 mg GAE/100 g extract (E3) respectively. The antioxidant activity (% inhibition) of the extract was 38,035 ± 0,252 % (E1), 52,502 ± 6,225 % (E2) and 57,261 ± 0,505 % (E3). Ascorbic acid as a positive control of antioxidant activity showed much higher % inhibition than the sample with 78,055 ± 0,756 %. As the best extract from antioxidant activity, E2 and E3 have inhibited collagenase activity with 51,809 ± 0,164 % and 52,212 ± 0,735 % at 20μg/mL respectively. Conclusion: In general the (T. hempricii) extract can be used as a potential active ingradient for anti-wrinkles cosmetic.

10aAnti-wrinkle10aAntioxidant10aCollagenase10aSeagrass10aThalassia hempricii1 aZakiah, Kiki1 aAnwar, Effionora1 aNurhayati, Tati uhttp://fulltxt.org/article/669