02661nas a2200241 4500008004100000245010700041210006900148260001600217300001200233490000700245520193500252653001802187653001702205653000802222653001202230653001902242100003102261700001902292700003102311700002202342700002002364856003502384 2018 eng d00aAntibacterial and Cytotoxic Activities of Sponges Collected off the Coast of Togean Islands, Indonesia0 aAntibacterial and Cytotoxic Activities of Sponges Collected off  cAugust 2018 a988-9920 v103 a

Context: Marine sponges (Porifera: Demospongia) have astonishing structural diversity and broad biological activities. Aims: To evaluate the antibacterial and cytotoxic activities of five sponges collected off the coast of Togean Islands, Indonesia, identified as Spheciospongia inconstan, Melophlus sarasironum, Oceanapia amboinensis, Biemna sp and Axinella sp. Methods and Material: All dried sponges materials were extracted by maceration method using methanol and then evaporated by the rotary evaporator to obtain viscous extracts. The determination of antibacterial activity was performed by well agar diffusion method against Staphylococcus aureus and Escherichia coli while the cytotoxic activity was determined by MTT methods on human breast adenocarcinoma (MCF-7) and human colon colorectal carcinoma (HCT-116), followed by determination of the apoptosis mechanism by Annexin V-FTIC assay. Results: M. sarasinorum and Axinella sp showed strong inhibition against S.aureus and E.coli with the diameter of inhibition of 14.21 ± 0.92 mm and 14.36 ± 0.92 mm, and 10.01 ± 2.65 mm and 12.07 ± 1.54 mm, respectively. Moreover, they also exhibited potent cytotoxicity on HCT-116 with IC50 values of 0.002 and 8.518 μg/mL, respectively. Meanwhile, on MCF-7, only M. sarasinorum showed moderate inhibition with an IC50 value of 87.35 μg/mL. Annexin V-FTIC assay clearly showed that the cytotoxic mechanism of M. sarasinorum and Axinella sp on HCT-116 and MCF-7 was via apoptosis induction. Conclusion: The sponges of M. Sarasinorum and Axinella sp are undergoing further analysis to identify the active constituents which could be developed as potential antibacterial and anticancer agents.

10aAntibacterial10aCytotoxicity10aMTT10aSponges10aTogean Islands1 aZubair, Muhammad, Sulaiman1 aLallo, Subehan1 aPutra, Masteria, Yunovilsa1 aHadi, Tri, Aryono1 aJantan, Ibrahim uhttps://phcogj.com/article/683