02786nas a2200301 4500008004100000245010700041210006900148260001600217300001400233490000700247520182700254653001702081653002602098653003102124653001502155653001402170100003202184700001802216700002902234700002902263700002902292700003202321700002002353700002802373700002302401700002502424856003502449 2018 eng d00aIn vitro Evaluation of Seaweed Gracilaria verrucosa for Cytotoxic Activity against Cervical HeLa Cells0 aIn vitro Evaluation of Seaweed Gracilaria verrucosa for Cytotoxi cAugust 2018 a1007-10110 v103 a

Background: Seaweed macroalgae of Gracilaria verrucosa has been known to have a potent anticancer activity, however the cytotoxicity against cervical cancer has not been explored further. Objective: This study aims to utilize Indonesia’s marine resource which is focused on seaweed macroalgae G. verrucosa as a future anti-cervical cancer agent. Materials and Method: Seaweed G. verrucosa originated from Labuan Aji beach, Nusa Tenggara Barat, Indonesia, extracted, macerated, and fractionated into four organic solvents of different polarity, consisting of hexane, ethyl acetate, chloroform and ethanol. Then, the macroalgae extracts are diluted into 8 different concentrations. Afterwards, in vitro anticancer activity evaluation of hexane, ethyl acetate, chloroform and ethanol extracts of G. verrucosa against cervical HeLa cells were conducted by MTT cell proliferation assay. Triplo mechanism is also applied in this study to increase the accuracy of the results. The anticancer activity is measured using IC50 value. Results: The four concentrated extracts G. verrucosa showed cytotoxicity against cervical HeLa cells. The greatest anticancer activity is depicted by hexane extract with an IC50 of 14.94 μg/mL, followed by chloroform (IC50 15.74 μg/mL), ethyl acetate (IC50 16.18 μg/mL), and ethanol (IC50 19.43 μg/mL). Conclusion: Our results clearly indicate that hexane, ethanol, chloroform, and ethyl acetate extracts of seaweed G. verrucosa can be further developed to be anti-cervical cancer agents, with hexane extract displaying the greatest cytotoxic effect.

10aCytotoxicity10aGracillaria verrucosa10aHeLa cervical cancer cells10aIC50 value10aMTT Assay1 aDewi, Micheylla, Kusumaning1 aArsianti, Ade1 aZagloel, Cut, Raisya Zah1 aAziza, Yully, Astika Nug1 aKurniasari, Kartika, Dwi1 aMandasari, Baiq, Kirana Dya1 aMasita, Riathul1 aZulfa, Futihati, Ruhama1 aAzizah, Norma, Nur1 aPutrianingsih, Rista uhttps://phcogj.com/article/706