02132nas a2200229 4500008004100000245016700041210006900208260001600277300001400293490000700307520134800314653001401662653002301676653001401699653002501713653003101738653001901769100002701788700003201815700002001847856003501867 2018 eng d00aIsolation of Cellulolytic Fungi and Utilization of Its Cellulolytic Activity for Microcrystalline Cellulose Preparation from Water Hyacinth (Eichhornia crassipes)0 aIsolation of Cellulolytic Fungi and Utilization of Its Celluloly cAugust 2018 a1082-10880 v103 a

Introduction: Microcrystalline cellulose is a cellulase derivative which usually used as a pharmaceutical excipient in the manufacturing of direct compression tablet. High concentration of cellulose is found in water hyacinth (about 60%) therefore it might be used in the manufacturing of microcrystalline cellulose. This study was aimed to obtain the best cellulolytic fungi, the best optimal conditions of enzymatic hydrolysis and comparing microcrystalline cellulose characteristics obtained from water hyacinth with microcrystalline cellulose standard, Avicel pH 101. Method: This study began with isolation of cellulolytic fungi, and then enzymatic hydrolysis of α-cellulose was done with cellulase enzymes extracted from cellulolytic fungi with various pH, temperature, duration and enzyme concentration. Microcrystalline obtained was characterized by XRD (X-Ray Diffraction) and then the result was compared with Avicel pH 101. Results: The results showed the best cellulolytic isolate was isolate 2 and its optimal hydrolysis conditions at pH 5, 30⁰C, for 1 h with 5 mL (5% v/v) enzyme. Based on the pattern of diffraction there was a similarity between microcrystalline cellulose of enzymatic hydrolysis result compared with Avicel pH 101.

10aCellulase10aCellulolytic fungi10aCellulose10aEnzymatic hydrolysis10aMicrocrystalline cellulose10aWater hyacinth1 aMurti, Mitayani, Wahyu1 aSudarsono, Monica, Angeline1 aSuryadi, Herman uhttps://phcogj.com/article/716