02738nas a2200277 4500008004100000245010700041210006900148260001600217300001400233490000700247520187600254653002902130653001002159653000902169653002902178653000702207653000802214100003002222700002802252700003102280700002502311700002902336700003202365700002802397856003502425 2018 eng d00aPhytochemical Screening and Antimicrobial Studies in Leaf Extracts of Indigofera aspalathoides (Vahl.)0 aPhytochemical Screening and Antimicrobial Studies in Leaf Extrac cAugust 2018 a1208-12150 v103 a

Introduction: In traditional Indian herbal medicine, the plant Indigofera aspalathoides (Vahl.) has been used to treat various human ailments. Methods and Results: Various phytochemical compounds (Rf value) such as Tannin (1.14), Flavonoid (1.14), Saponin (0.87), Sterol (0.88) and Phenol (0.86) were found in the leaf extract. The antimicrobial effects of the phytoconstituents were examined for three bacterial and fungal species. The highest anti-bacterial and antifungal activities were found in flavonoid compound, where the maximum zone of inhibition was recorded in Staphylococcus aureus 18mm compared to positive control chloramphenicol (24mm). 13mm observed in A. flavus and A. ochraceous where positive control streptomycin exhibits 18mm. GC-MS analysis revealed the presence of three major compounds in 8α (2H)-Phenanthrenol, 7-ethenyldodecahydro-1,1,4α, 7-tetramethyl-, acetate, [4αs(4αα, 4bα, 7α, 8αα, 10αα)], Benzoic acid, 4-methyl- , 2-oxo-2- phenylethyl ester and Cyclohexanol, 5-methyl-2-(1-methylethyl)-, [1S-(1α,2α,5α)] in the crude extracts. The preparative HPLC analysis proved the presence of single peak from the crude flavonoid compounds, where the absorption maximum was between 207-290nm exhibited by the UV spectrum analysis. FTIR spectrum confirmed the presence of amide group, phenol group, carboxylic acid, alkynyl, alkene and aromatic ring. NMR studies proved the presence of phenyl group, methyl group and H2C-CH group and their molecular weight recorded as 353 through the mass spectrum analysis. Conclusion: The identified compound considered as the vital compound to design the “green antimicrobial drugs”.

10aAntimicrobial activities10aGC-MS10aHPLC10aIndigofera aspalathoides10aMS10aNMR1 aKumar, Tamilarasan, Tamil1 aSalique, Seeni, Mohamed1 aIlyas, Mohamed, Hussain Mu1 aThajuddin, Nooruddin1 aPanneerselvam, Annamalai1 aPadusha, Mohamed, Khan Syed1 aJahangir, Hussain, Syed uhttps://phcogj.com/article/719