02492nas a2200217 4500008004100000245007700041210006900118260001600187300001400203490000700217520187600224653001102100653002002111653002102131653001102152653001302163100002302176700001602199700002402215856003502239 2018 eng d00aEffect of Lutein on Cytochrome P450 (Isoform CYP3A4) - An in vitro Study0 aEffect of Lutein on Cytochrome P450 Isoform CYP3A4 An in vitro S cAugust 2018 a1093-10950 v103 a

Background: Lutein is a carotenoids vitamin rich in many fruits and vegetables and also available in multivitamin products. It is consumed for its effect on eye disease, cancer, diabetes and other health conditions. Recently, herbal preparations are increasingly used in healthcare systems and concomitant administration of synthetic medications may cause pharmacokinetic or pharmacodynamic interactions leading to very serious medical problems. Understanding the ability of herbal extracts and preparations to modulate the metabolizing enzymes can help the health system for proper treatment of patients and thereby can avoid many adverse effects associated with it. The aim of the study was to find the effect of lutein isolated from Tagetes erecta L on cytochrome P450 isoform CYP3A4. Materials and Methods: The different concentrations of lutein (5 – 100μg/ml), potassium phosphate buffer, CYP450 reagent and substrate 7-Benzyloxy-4-trifluoromethylcoumarin (BFC) were added to a 96-well plate. The fluorescent intensities of the products were measured by Perkin Elmer Enspire fluorescence reader using an excitation and emission wavelength of 405 nm and 460 nm, respectively to examine the effect of lutein on Cytochrome P 450 isoform CYP3A4 and the IC50 was calculated by plotting concentrations of lutein against the corresponding percent inhibition. Results: All the tested concentrations of lutein showed potent inhibition against CYP3A4 in a dose – dependent manner. The IC50 value was found to be 35.27μg/ml. Conclusion: The inhibitory effect of lutein indicates the possibilities of herb-drug interaction if it is co – administered with prescribed drugs that are normally metabolised by CYP3A4 enzyme.

10aCYP3A410aCytochrome P45010aInhibitory assay10aLutein10aMarigold1 aMeenapriya, Murthy1 aAnitha, Roy1 aLakshmi, Thangavelu uhttps://phcogj.com/article/720