02717nas a2200229 4500008004100000245012400041210006900165260001600234300001400250490000700264520200300271653000602274653002202280653002702302653001202329653002602341653002102367653002102388100002602409700001702435856003502452 2018 eng d00aIdentification of Phytoconstituents in Lawsonia inermis Linn. Leaves Extract by GC-MS and their Antibacterial Potential0 aIdentification of Phytoconstituents in Lawsonia inermis Linn Lea cAugust 2018 a1101-11080 v103 a

Background: Plant extracts contains multiple active constituents which leads to the production of new drugs from plants and chemicals derived from various parts of plants. The objective of present study was to investigate the GC-MS analysis and antibacterial activity of L. inermis leaves extracts. Material and Methods: Crude methanol extract and its fractions were tested for the presence of active phytochemicals and GC-MS analysis of hexane; ethyl acetate and aqueous methanol fractions was performed. Antimicrobial activity against six bacterial strain’s Escherichia coli, Staphylococcus aureus, Bacillus subtelis, Salmonella typhi, Klebsiella and Pseudomonas aeruginosa was also tested. Results: Phytochemical screening of extract confirmed the presence of carbohydrates, glycosides, quinones, steroids and phenol. In GC-MS chromatograms, 56, 108 and 19 peaks were obtained and out of these, 13, 17 and 7 compounds were identified in hexane, ethyl acetate and aqueous methanol fractions, respectively. Conclusion: For best of our knowledge in L. inermis leaves extract, Celidoniol and Vitamin E has not been reported earlier in hexane fractions. While 2, 3 dihydrobenzo furan, 1-H indole -1, 3 (2H)-dione, 1 (3H)-Isobenzofuranone, 1H Isoindole-1, 3 (2H) Dione, Napthelene, 2 ethoxy, 2 (4H) Benzofuranone, Vitamin E, Benzene, 1 isocyano 4 methyl are also identified for the first time in ethyl acetate fraction. Also, in aq. Methanol fraction 1(3H)-isobenzofuranone, Squalene and Vit E were not previously identified. Highest antibacterial activity was confirmed in crude methanol extract which might be due to all the antibacterial compounds present in its fractions. The present study helped in identifying phytoconstituents present in the extract and its fractions which are responsible for various biological and antibacterial activities.

10a110a4 Napthelenedione10aAntimicrobial activity10aLawsone10aLawsonia inermis Linn10aMedicinal plants10aMethanol extract1 aSharma, Ritesh, Kumar1 aGoel, Anjana uhttps://phcogj.com/article/722