02566nas a2200265 4500008004100000245006100041210006100102260001600163300001400179490000700193520179100200653001201991653001902003653002302022653001302045653001802058100003302076700001702109700001702126700003302143700002802176700002902204700003202233856003502265 2018 eng d00aWithaferin A attenuates Alcohol Abstinence Signs in Rats0 aWithaferin A attenuates Alcohol Abstinence Signs in Rats cAugust 2018 a1190-11950 v103 a

Background: Withania somnifera (WS) have been reported to inhibit acquisition and expression conditioned place preference, self-administration and withdrawal anxiety of psychostimulants. In the present work, we have assessed the effect of withaferin A on somatic and affective symptoms of ethanol withdrawal syndrome in rats. Methods: Animals had given free access to ethanol uninterrupted for 21 days through liquid diet. Withaferin A (5, 10 and 20 mg/kg) was injected (ip) either during the development of ethanol dependence phase (days 15 – 21 or 30 min before ethanol withdrawal assessment. Withdrawal signs characterized by changes in somatic signs were measured in the open field followed by evaluation of anxiety parameters, locomotion, and depressive behavior. Results: Withaferin A treatment 30 min before 24 h postethanol withdrawal assessment did not alter the scores of somatic behavioral signs in ethanol abstinence animals. However, withaferin A (10 and 20 mg/kg, ip) from day 15-21 prevented the ethanol withdrawal-induced elevated scores of somatic behaviors, hyperlocomotion, depressive behavior, and anxiety. Withaferin A treatment did not influence the blood ethanol levels in dependent and withdrawn animals. However, withaferin A administration attenuated the elevated plasma corticosterone and ACTH levels in ethanol-withdrawn rats, suggesting withaferin A induced anti-stress effect and stabilization of HPA axis activity could have facilitated the inhibitory effect of withaferin A on ethanol withdrawal syndrome. Conclusion: The finding supports further investigation of withaferin A and other bioactive components of WS in alcohol addiction.

10aAnxiety10aCorticosterone10aEthanol withdrawal10aHPA axis10aWithaferin A.1 aKotagale, Nandkishor, Ramdas1 aKedia, Ankit1 aGite, Rupali1 aRahmatkar, Shubham, Nilkanth1 aGawande, Dinesh, Yugraj1 aUmekar, Milind, Janraoji1 aTaksande, Brijesh, Gulabrao uhttps://phcogj.com/article/739