02511nas a2200217 4500008004100000245019900041210006900240260001800309300001200327490000700339520171600346653002302062653004302085653004702128653001802175100001802193700002002211700002202231700000502253856003502258 2018 eng d00aComparison between High Performance Thin Layer Chromatography and High Performance Liquid Chromatography Methods for Determination of Rubraxanthone in the Stem Bark Extract of Garcinia cowa Roxb0 aComparison between High Performance Thin Layer Chromatography an cNovember 2018 as42-s470 v103 a

Objectives: To develop simple, rapid, accurate methods for determination of rubraxanthone in the stem bark extract of Garcinia cowa using High Performance Thin Layer Chromatography (HPTLC) and High Performance Liquid Chromatography (HPLC). Methods: The HPTLC method was performed on aluminum plate precoated with silica gel 60 F254 using Chloroform: Ethyl acetate: Methanol: Formic acid (88:2:2:8) as a developing system. Quantification was achieved using densitometric measurements at 243 nm. The HPLC method involved a 5 μm C18 column and an isocratic solvent using 0.4% formic acid: methanol (12:88) with a flow rate 1 mL minute-1. Quantitation was also achieved with ultraviolet detection at 243 nm based on peak area. All necessary validation tests for both methods were done for their comparison. The results obtained by these two different quantification methods were compared by Tukey’s-test. Results: Both assays provided good linearity, accuracy, precision, specificity and limits of detection and quantitation for determination of rubraxanthone in The Stem Bark extract of G. cowa. Conclusion: Both methods revealed reasonable validation parameters concerning linearity, accuracy, precision, specificity and limits of detection and quantitation. A statistical comparison of the quantitative analysis of rubraxanthone in extract did not show any statistically significant difference between two analysis methods. As both methods were found to be equal, they therefore can be used for the analysis of rubraxanthone in the Stem Bark extract of G. cowa.

10aGarcinia cowa Roxb10aHigh Performance Liquid Chromatography10aHigh performance Thin layer Chromatography10arubraxanthone1 aSusanti, Meri1 aIbrahim, Sanusi1 aHarahap, Yahdiana1 a uhttps://phcogj.com/article/755