02660nas a2200241 4500008004100000245013700041210006900178260001700247300001200264490000700276520193300283653001102216653001502227653000902242653000902251653001302260653001502273100002102288700002502309700001902334700003002353856003502383 2019 eng d00aComparative GC-MS Analysis of Bioactive Phytochemicals from Different Plant Parts and Callus of Leptadenia reticulata Wight and Arn.0 aComparative GCMS Analysis of Bioactive Phytochemicals from Diffe cJanuary 2019 a129-1400 v113 a

Aim: The aim of this study is identification and comparative analysis of bioactive phytochemicals present in methanol and ethyl acetate extracts of different plant organs and callus of Leptadenia reticulata by GC–MS technique. Methodology: The shade dried plant samples (leaves stem and root) and callus produced from leaf of L. reticulata were powdered and then sequentially extracted in methanol and ethyl acetate solvents. Total eight extracts were prepared which were Methanolic Leaf Extract (MLE), Methanolic Stem Extract (MSE), Methanolic Root Extract (MRE), Methanolic Callus Extract (MCE), Ethyl Acetate Leaf Extract (EALE), Ethyl Acetate Root Extract (EARE), Ethyl Acetate Stem Extract (EASE) and Ethyl Acetate Callus Extract (EACE). Then, each of the extracts was further subjected to Gas Chromatography– Mass Spectrometry (GC–MS) analysis. Results: The GC–MS analysis of the eight extracts revealed the presence of 77 different types of high and low molecular weight phytochemicals and bioactive compounds in varying quantities. Some of the phytochemicals detected for first time in L. reticulata are γ-sitosterol, Campesterol, Pristane, Hexahydrofarnesol, Stearic acid, Arachidic acid, Coniferyl alcohol, n-Tetracosanol-1, Ascorbic acid 2,6-dihexadecanoate, (2S,3S)-3,7,4’-Trihydroxy-5-methoxy-6-methylflavanone etc. These chemical compounds are considered biologically active and pharmacologically important. Conclusion: This study gives a detailed comparison of detection and identification of various bioactive phytochemicals from different plant parts (leaves, stem and root) and callus of L. reticulata. This provides a basis for the biological and biochemical characterization of some newly detected biologically and pharmacologically important phytochemical components from this plant.

10aCallus10aFatty Acid10aRoot10aStem10aSteroids10aTerpenoids1 aGodara, Priyanka1 aDulara, Bunty, Kumar1 aBarwer, Neelam1 aChaudhary, Navneet, Singh uhttps://phcogj.com/article/789