02171nas a2200277   4500008004100000245010000041210006900141260001200210300001200222490000600234520132900240653001301569653001201582653001101594653003801605653001901643653001801662100003201680700002801712700003201740700001801772700002001790700002201810700002701832856003401859       2015                        eng d00aAnti-diabetic Properties of Thymoquinone is unassociated with Glycogen Phosphorylase Inhibition0 aAntidiabetic Properties of Thymoquinone is unassociated with Gly  c01/2015  a406-4100 v73 a<p style="text-align: justify;"><strong>Background:</strong> <em>Nigella sativa</em> L. (Black seed), is commonly used by traditional healers as a remedy for more than four thousand years. The antidiabetic property of <em>N. sativa</em> seeds oil is attributable to the presence of Thymoquinone (TQ). On the other hand many studies have been designed to investigate the possible effects of the TQ in Streptozotocin (STZ) and nicotinamide (NA)-induced diabetes in rats. <strong>Aim of the study: </strong>The aim of this study was to elucidate the mechanisms underlying the glucose lowering effects of thymoquinone. <strong>Methods:</strong> <em>In vitro</em> and <em>in silico</em> using glycogen phosphorylase (GPa) enzyme assay and docking tools were used. <strong>Results:</strong> Oral administration of TQ for 60 days, dose dependently improved the glycemic status in STZ-NA induced diabetic rats. GPa activity was measured in the direction of glycogen synthesis by the release of phosphate from glucose-1-phosphate. TQ at a concentration of 0.05 Mm inhibits GPa activity by only 14.9%. <strong>Conclusion:</strong> These results show that TQ at 60 mg/kg b.w is associated with potential antihyperglycemic effects. Furthermore, anti-diabetic properties of TQ are unassociated with glycogen phosphorylase inhibition.</p>10aDiabetes10aDocking10aEnzyme10aGlycogen phosphorylase inhibition10aStreptozotocin10aThymoquinone.1 aEl-Ameen, Nahid, Mahmoud Ha1 aTaha, Manal, Mohamed El1 aAbdelwahab, Siddig, Ibrahim1 aKhalid, Asaad1 aElfatih, Fatima1 aKamel, Mona, Awad1 aSheikh, Bassem, Yousif  uhttps://phcogj.com/article/83