02620nas a2200229 4500008004100000245012000041210006900161260001800230300001200248490000700260520189200267653002802159653001502187653002502202653001902227653000902246653002302255653003102278100002102309700002502330856003502355 2019 eng d00aPhytochemical Constituents and Free Radical Scavenging Activity of Madang Gatal (Schima wallichii) Choisy Stem Bark0 aPhytochemical Constituents and Free Radical Scavenging Activity  cFebruary 2019 a395-3990 v113 a

Introduction: Madang gatal (Schima wallichii) Choisy is one of typical Indonesian plants that used traditionally as insomnia and hypertension drug. Background: The environment is highly polluted by free radicals which may contribute to the aging process of tissue and cause chronic diseases. The aims of the research were to study the phytochemical constituents and free radical scavenging activity of Schima wallichii stem bark. Materials and Methods: Extraction of S.wallichii stem bark was carried out by maceration method using methanol and then the methanol extract was partitioned using n-hexane, ethyl acetate and butanol successively. The methanol extract was then screened to determine the presence of different classes of secondary metabolites. The ethyl acetate fraction was subjected to silica column chromatography followed by exclusion chromatography on sephadex LH-20 to yield two partially purified isolates, SA and SE4. FTIR and GC-MS analyses indicated SA asa mixture of pentadecanoic acid, 14-methyl-, methyl esterand patchouli alcohol, while SE4 was made up predominantly of pentadecanoic acid, 14-methyl-, 13-hydroxy methyl ester. Free radical scavenging activity was performed by using2,2-diphenyl-1-picrylhydrazyl (DPPH) radical oxidation method. Results: The phytochemical screening indicated the presence of alkaloids, flavonoids, tannins, saponins, terpenes and quinones. All tested samples, methanol, n-hexane, ethyl acetate and butanol extracts showed strong antioxidant activity with IC50 values of 8.58, 17.36, 8.17 and 8.79 μg/mL, respectively. The isolates showed strong antioxidant activity with SE4 exhibiting an IC50 value of about 5.5 times stronger than quercetin used as a standard antioxidant.

10a13-hydroxy methyl ester10a14-methyl-10aantioxidant activity10aChromatography10aDPPH10aPentadecanoic acid10aSchima wallichii stem bark1 aWidiyarti, Galuh1 aFitrianingsih, Winda uhttps://phcogj.com/article/832