02456nas a2200229 4500008004100000245013600041210006900177260001800246300001200264490000700276520172500283653001602008653002102024653001902045653002702064653002402091100002202115700002102137700000502158700002802163856003502191 2019 eng d00aAntioxidant and Tyrosinase Inhibitor Activities of Ethanol Extracts of Brown Seaweed (Turbinaria conoides) as Lightening Ingredient0 aAntioxidant and Tyrosinase Inhibitor Activities of Ethanol Extra cFebruary 2019 a379-3820 v113 a

Background: Turbinaria conoides is one of abundant brown macroalgae in Indonesian oceans contains phenolic derivatives. Phloroglucinol is a multifunctional phenolic derivative in brown algae. Phloroglucinol has been known to have activity inhibitor tyrosinase, because phloroglucinol is copper chelating agent. The antioxidant and the tyrosinase inhibitor activities are parameters of the skin lightening active ingredient. The mechanism of tyrosinase inhibitors is to decrease skin pigmentation by inhibit the catalytic steps of the enzyme to the pigmentation associated with melanin production in the pathway of melanogenesis. Antioxidant activity protects skin against oxidative stress mediated by UVR. Numerous report have determine phenolic compound of T. conoides as antioxidant but none is reported as tyrosinase inhibitor. Objective: The objective of this study is to determine the activities of T. conoides as antioxidant and tyrosinase inhibitor. Methods: Turbinaria conoides were extracted with ethanol with different concentration (30%, 50% and 70%) by maceration method. Crude ethanolic extracts of T. conoides were determined its total phenolic content, antioxidant activity (DPPH) and tyrosinase inhibitor. Results: E50 showed the highest total phenolic content (27.63±1.05 mg PGE/g extract). Antioxidant and tyrosinase inhibitor activities of E50 showed IC50 value of 215.96 μg/mL and 188.85 μg/mL, respectively. Conclusion: Ethanol extract of Turbinaria conoides is potential as antioxidant agent and tyrosinase inhibitor.

10aAntioxidant10aLightening agent10aPhloroglucinol10atotal phenolic content10aTurbinaria conoides1 aSari, Diani, Mega1 aAnwar, Effionora1 a1 aArifianti, Ayun, Erwina uhttps://phcogj.com/article/847