02387nas a2200241 4500008004100000245015900041210006900200260001800269300001200287490000700299520159600306653001801902653002801920653001201948653001801960653001401978100002701992700001702019700002902036700002002065700002502085856003502110 2019 eng d00aPhytochemical Composition, Proximate Analysis and Antimicrobial Screening of the Methanolic Extract of Diospyros mespiliformis Hochst ex a. Dc (ebenaceae)0 aPhytochemical Composition Proximate Analysis and Antimicrobial S cFebruary 2019 a362-3680 v113 a

Aim: Diospyros mespiliformis is one plant used by the natives for the treatment of certain diseases including wounds. In this present study, preliminary screening of the methanolic leaf extract of D. mespiliformis was carried out for its phytochemical and proximate constituents in addition to investigating its antimicrobial activity against four bacteria species. Methodology: Preliminary phytochemical and proximate analysis were carried out using standard methods. The antimicrobial activity was conducted using the whole in-plate and broth serial micro dilution assays on two Gram positive bacteria (Staphylococcus aureus, Salmonella typhymurium) and two Gram negative bacteria (Pseudomonas aeruginosa and Escherichia coli). Results: The phytochemical screening showed the presence of alkaloids, tannins, saponins, glycosides, anthraquinones, flavonoids and volatile oil. Similarly, preliminary proximate analysis of the root, bark and leaf extracts of D. mespiliformis exerts revealed the presence of carbohydrate, crude protein, moisture, lipid and nitrogen, ash and fibre. The extract demonstrated greater inhibitory activity on P. aeruginosa, S. aureus and E. coli (MIC=156.25 μg/ml) than on S. typhymurium (MIC=312.5 μg/ml). Conclusion: These findings revealed that the crude methanolic extract of D. mespiliformis and its fractions demonstrated broad spectrum antimicrobial activity in a dose dependent manner.

10aAntimicrobial10aDiospyros mespiliformis10aExtract10aPhytochemical10aProximate1 aEbbo, Abdullahi, Aliyu1 aSani, Dahiru1 aSuleiman, Mohammed, Musa1 aAhmed, Abubakar1 aHassan, Adamu, Zoaka uhttps://phcogj.com/article/850