02330nas a2200277 4500008004100000245010800041210006900149260001800218300001200236490000700248520144800255653002001703653001501723653001901738653001801757653002301775653003801798100002801836700003001864700002301894700002201917700002401939700002801963700002601991856003502017 2019 eng d00aPhytochemical, in vitro Antioxidant and in vivo Safety Evaluation of Leaf Extracts of Tragia plukenetii0 aPhytochemical in vitro Antioxidant and in vivo Safety Evaluation cFebruary 2019 a338-3450 v113 a

Objective: To investigate the phytochemical properties, in vitro antioxidant and in vivo safety profile of leaf extracts of Tragia plukenetii (TP). Methods: TP leaves were obtained from the south part of India (Guntur District, Andhra Pradesh) and it was extracted with different solvents (benzene extract (BE), chloroform extract (CE) and methanolic extract (ME)). These TP extracts were analyzed for the in vitro antioxidant activity by DPPH reducing power, β-carotene-linoleic acid complex and iron chelation assays followed by in vivo acute oral and dermal toxicities using Swiss mice and Wistar rats respectively. Results: The present study results revealed ME exhibited an effective and powerful antioxidant activity when compared to a standard antioxidant, butylated hydroxytoluene (BHT). ME was found to be effective in DPPH, β-carotene-linoleic acid complex and iron chelation assays respectively. In vivo acute oral toxicity study revealed that mice treated with up to 5000 mg/kg of BE, CE and ME did not show any signs of toxicity. Furthermore, similarly, acute dermal toxicity study demonstrated that BE, CE and ME did not exhibit any signs of dermal toxicity up to 1000 mg/kg in rats. Conclusion: TP extracts possess an excellent antioxidant activity with a devoid of any signs of acute oral and dermal toxicities.

10aDermal toxicity10aDPPH assay10aIron chelation10aOral toxicity10aTragia plukenetii.10aβ-carotene-linoleic acid complex1 aBonam, Srinivasa, Reddy1 aManoharan, Sathish, Kumar1 aPandy, Vijayapandi1 aRaya, Anji, Reddy1 aNadendla, Rama, Rao1 aJagadeesan, Manjunathan1 aBabu, Ankem, Narendra uhttps://phcogj.com/article/852