02432nas a2200205 4500008004100000245013400041210006900175260001400244300001200258490000700270520176400277653002002041653001702061653003302078653001802111653002002129100002602149700001602175856003502191 2019 eng d00aAnti-inflammatory Effect of the Aqueous Fruit Pulp Extract of Tamarindus indica Linn in Lipopolysaccharide-Stimulated Macrophages0 aAntiinflammatory Effect of the Aqueous Fruit Pulp Extract of Tam cJuly 2019 a669-6730 v113 a

Aim: The aim of the present study was to evaluate the effect of the aqueous fruit pulp extract of Tamarind indica Linn on NO production and iNOS expression in LPS stimulated RAW 264.7 macrophages. Material and Method: The efficacy of tamarind extract on nitric oxide production was determined using RAW macrophages. RT - PCR was used to examine the expression of the iNOS gene in activated macrophages. The Statistical analysis for multiple comparisons was evaluated by one way ANOVA followed by the Dunnett’s test when significant differences were detected. The data were considered to be statistically significant at p < 0.001, p < 0.01 and p < 0.05. Results: LPS stimulated RAW macrophages strongly up regulated the iNOS gene expression levels. The iNOS levels were significantly suppressed in the presence of different concentrations of tamarind extract, compared to LPS treatment alone. The tamarind extract also exhibited dose – dependent decrease in the production of NO. The IC50 was found to be 35.69 μg/ml. LPS stimulated group showed 89.61 ± 0.47 % of NO. Conclusion: Nitric oxide production is found to be more in conditions such as periodontitis, oral squamous cell carcinoma and many other diseases. This study could prove the ability of tamarind fruit pulp extract to inhibit the production of nitric oxide and the iNOS gene expression. Hence, Tamarind indica Linn pulp extract may be used as a good anti-inflammatory agent in periodontitis as well as in conditions associated with over production of nitric oxide in different cancers such as oral squamous cell carcinoma.

10aiNOS expression10aNitric oxide10aOral squamous cell carcinoma10aPeriodontitis10aTamarind indica1 aLeya, Mathews, Meriam1 aAnitha, Roy uhttps://phcogj.com/article/902