02437nas a2200241 4500008004100000245013900041210006900180260001400249300001200263490000700275520162400282653002601906653002601932653005301958653001802011653002802029653001402057653003102071653002302102100001802125700001702143856003502160 2019 eng d00aIn vitro Antidiabetic Activity of Methanolic Leaf Extract of Indianthus virgatus (Roxb.) Suksathan and Borchs by Glucose Uptake Method0 aIn vitro Antidiabetic Activity of Methanolic Leaf Extract of Ind cJuly 2019 a674-6770 v113 a

Medicinal plants play a key role to cure many diseases from time immemorial. The usage of medicinal plants in traditional medicinal system is the vital process of India. Diabetes Mellitus is a systemic metabolic disease characterized by hyperglycemia, abnormal elevated levels of lipid and fat in blood and hypoinsulinaemia. The current epidemic of diabetes indicates the need of proper and effective medications that are limited in their potency to have many side effects. Thus, introduction of alternative and complementary medicine is now in picture. Objective: The main objective of this work is to evaluate the in vitro anti diabetic activity of methanolic extracts of Indianthus virgatus (Roxb.) Suksathan and Borchs in skeletal muscle cell line. Methods: The in vitro cytotoxicity was performed for leaf extract (Methanol) on L-6 (Rat skeletal muscle) cell line to find toxic concentration of the leaf extract by MTT assay. Glucose uptake activity of test substance was determined in differentiated L-6 cells. Results: In Glucose uptake assay, Methanol Extract exhibited moderate toxicity to skeletal muscle cell line and glucose uptake assay it shows dose dependent glucose uptake. Glucose uptake rate increased with the increasing concentration of the leaf extract. Conclusion: The results of the current study clearly demonstrated the antidiabetic potency of methanolic leaf extract obtained from Indianthus virgatus (Roxb.) Suksathan and Borchs. under in vitro model.

10aAntidiabetic activity10aIn vitro cytotoxicity10aIndianthus virgatus (Roxb.) Suksathan and Borchs10aL-6 cell line10aMethanolic leaf extract10aMTT Assay10aSkeletal muscle Cell Lines10aTherapeutic agents1 aDN, Sangeetha1 aRajamani, S. uhttps://phcogj.com/article/904