02346nas a2200229 4500008004100000245011700041210006900158260001300227300001200240490000700252520164400259653001601903653001701919653001401936653001701950653002501967100002101992700002502013700002302038700002002061856003502081 2019 eng d00aScreening of Tyrosinase Inhibitor, Antioxidant and Cytotoxicity of Dried Sea Cucumber from Tomini Bay, Indonesia0 aScreening of Tyrosinase Inhibitor Antioxidant and Cytotoxicity o cMay 2019 a555-5580 v113 a

Background: Indonesia, as a tropical country, is one of the important producers of sea cucumbers (beche-de-mer). Sea cucumber is a marine invertebrate that contains attractive bioactive secondary metabolites and these metabolites can be used for health as well as cosmetics. Objective: The aim of the study was to determine the activity of tyrosinase inhibitors, antioxidants, and cytotoxicity of sea cucumber methanolic extract. Methods: Dried sea cucumber samples were taken from Boalemo waters, Tomini Bay, Indonesia. Tyrosinase inhibitor assay was carried out spectrophotometrically using tyrosinase enzymes and L-DOPA as a substrate and antioxidant tests were carried out by DPPH method. Cytotoxicity test against human breast cancer cell line (T47D) was conducted using the MTT assay. Results: The study showed that Bohadschia vitiensis had the best tyrosinase inhibitor activity with IC50 value of 0.28 mg/ml. The DPPH free radical scavenging testing showed that all sea cucumbers had weak antioxidant activity. On the other hand, cytotoxicity assay revealed that several sea cucumbers had good cytotoxicity against T47D cells, where Holothuria atra and Bohadschia marmorata showed strong cytotoxicities with IC50 values of 23.0 and 28.1 ug/mL, respectively. Conclusion: Based on the study, it can be concluded that the dried sea cucumber from the Tomini bay region, Indonesia, has the potential to be developed as a source of tyrosinase inhibitors and cytotoxic agents.

10aAntioxidant10aCytotoxicity10aScreening10aSea cucumber10aTyrosinase inhibitor1 aNursid, Muhammad1 aMarraskuranto, Endar1 aKuswardini, Azizah1 aWinanto, Tjahyo uhttps://phcogj.com/article/918