02633nas a2200229 4500008004100000245011300041210006900154260001400223300001200237490000700249520190400256653002502160653001602185653002202201653002702223653002102250100002102271700002602292700002302318700002702341856003502368 2019 eng d00aAntioxidant Activity of Ethanolic Extract and Various Fractions from Green Tea (Camellia sinensis L.) Leaves0 aAntioxidant Activity of Ethanolic Extract and Various Fractions  cJuly 2019 a771-7760 v113 a

Background: Free radicals are one of the causes that can cause premature aging and degenerative disease. To overcome this problem, the body needs antioxidant intake. Green tea (Camellia sinensis L.) leaves are one of the plants known as antioxidant agent due to its flavonoids and phenolic compounds or better known as catechin compounds. Catechin is polar flavonoid compounds so it is necessary to separate it from non-polar compounds so their antioxidant activity becomes effective. Objective: This study aims to determine antioxidant activity of ethanolic extract of green tea leaves and its fractions namely ethyl acetate and water fraction, and measure the total flavonoid content, total phenolic content and catechin content. Materials and Methods: Green tea leaves extracted using maceration method with 96% ethanol. Fractionation was conducted using liquid-liquid extraction using a solvent of n-hexane, ethyl acetate and water. Screening of flavonoid and phenolic and antioxidant activity was performed against the ethanolic extract, ethyl acetate fraction and water fraction. Antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl method using ultravioletvisible spectrophotometry with ascorbic acid as standard. Results: The ethanolic extract, ethyl acetate fraction and water fraction contains flavonoids and phenolic compounds. The IC50 value of ethanolic extract, ethyl acetate fraction and water fraction were 9.017; 3.926 and 7.408 μg/mL consecutively. The ethyl acetate fraction also showed better antioxidant activity than ascorbic acid (4.855 μg/mL). Conclusion: The ethanolic extract, ethyl acetate fraction and water fraction showed very powerful antioxidant activity but ethyl acetate fraction has the best antioxidant activity.

10aantioxidant activity10aDPPH method10aEthanolic extract10aEthyl acetate fraction10aGreen tea leaves1 aIndarti, Karmika1 aApriani, Elsa, Fitria1 aWibowo, Agung, Eru1 aSimanjuntak, Partomuan uhttps://phcogj.com/article/921