02966nas a2200253 4500008004100000245011100041210006900152260001400221300001200235490000700247520218100254653001202435653002202447653002202469653002002491653002602511653002102537653002002558100003302578700002302611700002502634700001802659856003502677 2019 eng d00aEffect of Diallyl Disulphide (DADS) on Gluconeogenesis: A Study in Isolated Alloxan Induced Diabetic Liver0 aEffect of Diallyl Disulphide DADS on Gluconeogenesis A Study in  cJuly 2019 a777-7810 v113 a

Background: Coordinated regulation between cellular glucose uptake and endogenous glucose production is indispensable for maintenance of constant blood glucose concentrations. Liver contributes significantly by altering levels of hepatic glucose release, through controlling gluconeogenesis. Objective: To assess effect of Diallyldisulphide on hepatic gluconeogenesis by assessing gluconeogenic substrates and activity of regulatory enzyme glucose-6-phosphatase and to compare the Diallyldisulphide effect on gluconeogenesis with Metformin. Methodology: Wistar albino rats were categorized into four groups; group Inormal control. Group II, III and IV rats were administered single intraperitoneal injection of alloxan monohydrate 150 mg/kg body weight. Rats were maintained on rat diet and water adlibitum for 30 days. On 31st day rats were sacrificed and procured liver tissues. Each liver tissue cut into small slices and group-III and group-IV liver tissues were separately incubated with Diallyldisulphide 4 mg/g liver tissues and Metformin 4 mg/g liver tissues for 60 minutes at 37oC. The total amino acids and total keto acids levels were estimated after exposure. And post exposure effect on activity of glucose -6-phoshatase and transaminases were also estimated. Results: Alloxan induced diabetes resulted significant raise in total amino acids, total ketoacids, glucose -6-phosphatase and transaminases activities. Whereas incubation of liver tissues with diallyldisulphide significantly reduced total amino acids, total ketoacids, glucose-6-phosphatase, transaminases activities and ratio of total amino acids and ketoacids as compared to diabetic control rat liver tissues. Conclusion: Study suggested that diallyldisulphide suppressed gluconeogenesis as liver glucose-6-phosphatase activity is significantly decreased and precursors for gluconeogenesis were significantly reduced. Suggesting Diallyldisulphide has role in gluconeogenesis and can hold potentials for glucose homeostasis.

10aAlloxan10aDiabetes mellitus10aDiallyldisulphide10aGluconeogenesis10aGlucose-6-phosphatase10aTotal amino acid10aTotal keto acid1 aGoudappala, Prashanth, kumar1 aSukumar, Ethirajan1 aYogaraje-Gowda, C, V1 aRT, Kashinath uhttps://phcogj.com/article/932