TY - JOUR T1 - Two Triterpenoid Saponins with alpha-glucosidase Inhibitory Activity from Harpullia pendula Seed Extract JF - Pharmacognosy Journal Y1 - 2019 A1 - Marian Nabil A1 - Neveen S Ghaly A1 - Iman AA Kassem A1 - Mary H Grace A1 - Farouk R Melek KW - Harpullia pendula KW - Sapindaceae KW - Triterpenoid saponins AB -

Background: Harpullia pendula Planch (family Sapindaceae) is a small to medium rainforest tree native to Australia. Objective: This study aims to isolate triterpenoid saponins from H. pendula and test them as α-glucosidase inhibitors. Materials and Methods: The saponin compounds were obtained using variable chromatographic techniques and characterized by spectral analysis. Results: Two new triterpenoid saponins were obtained as an inseparable mixture from H. pendula methanolic seed extract. Their structures were determined as 3-O-β-D-glucopyranosyl-(1→2)-[α-L-arabinofuranosyl-(1→3)]-βD-glucuronopyranosyl22-OangeloylA1- barrigenol and 3-O-β-D-galactopyranosyl-(1→2)-[α-L-arabinofuranosyl-(1→3)]-β-Dglucuronopyranosyl 22-O-(2-methylbutyroyl)-A1 barrigenol, respectively. The triterpene part 22-O-(2-methyl butyroyl) A1-barrigenol has never been characterized before. The α- glucosidase inhibitory activity of the two saponin mixture was evaluated invitro and proved to exhibit strong activity with IC50 value equals to 13.3 ± 5.0 ppm and IC90 value equals to 21.5 ± 8.0 ppm. Conclusion: Two new saponins were characterized from their mixture and found to exhibit α-glucosidase inhibitory activity.

VL - 11 IS - 6s ER -