<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Onyekere Peculiar Feenna</style></author><author><style face="normal" font="default" size="100%">Odoh Uchenna Estella</style></author><author><style face="normal" font="default" size="100%">Ezugwu Christopher Obodike</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Phytochemical Analysis and Anti-diabetic Activity of Leaf extract of Psydrax horizontalis Schum and Thonn (Rubiaceae)</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Alloxan monohydrate</style></keyword><keyword><style  face="normal" font="default" size="100%">Diabetes</style></keyword><keyword><style  face="normal" font="default" size="100%">Extraction</style></keyword><keyword><style  face="normal" font="default" size="100%">Flavonoids</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">February 2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">95-102</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction: &lt;/strong&gt;Rubiacaeae is a large family of flowering plants of 630 genera with over 13000 species widely distributed in the tropical and warm regions of the world. The &lt;em&gt;Psydrax genus &lt;/em&gt;has been reported to have various pharmacological activities. Based on ethno-pharmacological information, &lt;em&gt;Psydrax horizontalis &lt;/em&gt;Schum. and Thonn. (Bridson) locally known as “&lt;em&gt;Akata-ike&lt;/em&gt;” in Nsukka is used in the management of diabetes in South-eastern Nigeria. However as at the time of this research, no previous work has been done to investigate its phytochemical constituents and anti-diabetic activity. &lt;strong&gt;Methods:&lt;/strong&gt; The methanol extract (ME) obtained by maceration was analysed for phytochemicals present using standard procedures. Alloxan monohydrate at 150 mg/Kg was used to induce diabetes. Acute toxicity test was done using the Lorke’s method. The normoglycemic and alloxan-induced groups of twenty animals each were treated orally with 100, 200 and 400 mg/Kg of ME; 5 mg/Kg of glibenclamide and 2 mL/kg of 3% Tween 80 as the controls. The fasting blood glucose concentrations were monitored at 0, 1, 3 and 6 h, as well as 1&lt;sup&gt;st&lt;/sup&gt;, 3&lt;sup&gt;rd&lt;/sup&gt; and 5&lt;sup&gt;th&lt;/sup&gt; day. &lt;strong&gt;Results:&lt;/strong&gt; Phytochemical screening revealed the presence of glycosides, alkaloids, tannins, flavonoids, steroids, saponins and terpenoids. Median lethal dose was greater than 5000 mg/kg. In the alloxan-induced diabetic rats, significant activity at &lt;em&gt;p&lt;/em&gt;&amp;lt;0.01 on the 6th hour was recorded at doses 100, 200 and 400 mg/Kg ME on day 3. &lt;strong&gt;Conclusion:&lt;/strong&gt; The ME of &lt;em&gt;Psydrax horizontalis&lt;/em&gt; possesses significant anti-diabetic activity in the alloxan-induced diabetic rats. The results obtained validate its traditional use in management of diabetes.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">95</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Onyekere Peculiar Feenna&lt;sup&gt;1,2,&lt;/sup&gt;*, Odoh Uchenna Estella&lt;sup&gt;1&lt;/sup&gt;, Ezugwu Christopher Obodike&lt;sup&gt;1&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Pharmacognosy and Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka. 410001. Enugu State, Nigeria.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Health Sciences, Hamburg University of Apllied Sciences, HAWHamburg, Germany.&lt;/p&gt;
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