<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Debasish Pradhan</style></author><author><style face="normal" font="default" size="100%">Gitanjali Tripathy</style></author><author><style face="normal" font="default" size="100%">Rakesh Kumar Pradhan</style></author><author><style face="normal" font="default" size="100%">Shaktiprasad Pradhan</style></author><author><style face="normal" font="default" size="100%">Soumyashree Rupambika Moharana</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Inhibition of MDR1 in mammary cell carcinoma reverses Multidrug Resistance by SOCS1</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Breast cancer</style></keyword><keyword><style  face="normal" font="default" size="100%">MDR1gene</style></keyword><keyword><style  face="normal" font="default" size="100%">Multidrug resistance</style></keyword><keyword><style  face="normal" font="default" size="100%">RNA interference.</style></keyword><keyword><style  face="normal" font="default" size="100%">SOCS1 gene</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2016</style></year><pub-dates><date><style  face="normal" font="default" size="100%">09/2015</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">8</style></volume><pages><style face="normal" font="default" size="100%">103-112</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Introduction&lt;/strong&gt;: Suppressors of cytokine signalling (SOCS1), a newly indentified antiapoptotic molecule is a downstream effector of the receptor tyrosine kinase-Ras signalling pathway. Current study has uncovered that SOCS1 may have wide and imperative capacities, particularly because of its close correlation with malignant tumors. &lt;strong&gt;Methods:&lt;/strong&gt; To investigate the impact of SOCS1 on MDR, we analyzed the expression of P-gp and SOCS1 by immunohistochemistry and found there was positive correlation between them. At that point we effectively interfered with RNA translation by the contamination of siRNA of SOCS1 into MCF7/ ADM breast cancer cell lines through a lentivirus, and the expression of the target gene was significantly inhibited. &lt;strong&gt;Results:&lt;/strong&gt; After RNAi the drug resistance was reduced altogether and the expression of MDR1 mRNA and P-gp in MCF7/ADM cell lines demonstrated a significant decrease. Likewise the expression of P53 protein increased in a statistically significant manner (p&amp;le;0.01) after RNAi exposure. Moreover, flow cytometry analysis uncovers that cell cycle and anti-apoptotic enhancing capacity of cells changed after RNAi treatment. &lt;strong&gt;Conclusion:&lt;/strong&gt; These outcomes proposed SOCS1 may take part in breast cancer MDR by managing MDR1 and P53 expression, changing cell cycle and enhancing the anti-apoptotic ability of cells.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">103</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Debasish Pradhan*, &lt;/strong&gt;&lt;strong&gt;Gitanjali Tripathy,&lt;/strong&gt; &lt;strong&gt;Rakesh Kumar Pradhan, Shaktiprasad Pradhan, Soumyashree Rupambika Moharana&lt;/strong&gt;&lt;/p&gt;

&lt;p style=&quot;text-align: justify;&quot;&gt;University Department of Pharmaceutical Sciences, Utkal University, Vani Vihar, Bhubaneswar, Odisha, INDIA.&lt;/p&gt;
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