<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Tutik Sri Wahyuni</style></author><author><style face="normal" font="default" size="100%">Adita Ayu Permanasari</style></author><author><style face="normal" font="default" size="100%">Aty Widyawaruyanti</style></author><author><style face="normal" font="default" size="100%">Hak Hotta</style></author><author><style face="normal" font="default" size="100%">Chie Aoki-Utsubo</style></author><author><style face="normal" font="default" size="100%">Achmad Fuad Hafid</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Antiviral Activity of Indonesian Medicinal Plants against Hepatitis B Virus</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Curcuma xanthorhiza</style></keyword><keyword><style  face="normal" font="default" size="100%">Hepatitis B Virus</style></keyword><keyword><style  face="normal" font="default" size="100%">Medicinal plants</style></keyword><keyword><style  face="normal" font="default" size="100%">Phyllanthus niruri</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">August 2020</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">1108-1114</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;Hepatitis B virus (HBV) infects more than 300 million people globally and is a common cause of liver cancer. Current therapy using reverse transcriptase inhibitors require long-term treatment and the potential risk of development of drug-resistant viruses remains an important issue to be considered. Hence, the development of new drugs is critical. Traditional medicinal plants used for the treatment of infectious diseases may provide a viable option for the discovery of anti-HBV drug candidates. &lt;strong&gt;Objective: &lt;/strong&gt;This study examined anti-HBV activity of 31 kinds of Indonesian plants. &lt;strong&gt;Materials and Methods: &lt;/strong&gt;Crude extracts of various part of plants, leaves and stem, were obtained using dichloromethane and ethanol solvent. The effect on viral entry was examined by determining levels of HBsAg expression in the supernatants of HBV-infected HepG2-NTCP cells by ELISA. The effect on HBV replication was determined by measuring HBV DNAs amounts in Hep38.7-Tet cells by quantitative real-time PCR.&lt;strong&gt; Results: &lt;/strong&gt;The extracts of &lt;em&gt;Phyllantus niruri&lt;/em&gt; leaves and &lt;em&gt;Curcuma xanthorrhiza &lt;/em&gt;showed reduction of strong HBsAg production from HepG2-NTCP cells with IC&lt;sub&gt;50&lt;/sub&gt; values of 170.48 and 270.51 μg/mL, respectively. Treatment of HepAD38.7-Tet cells with &lt;em&gt;P. niruri &lt;/em&gt;and &lt;em&gt;C. xanthorhiza &lt;/em&gt;at the highest concentration while avoiding cytotoxicity reduced extracellular HBV DNA levels by 70% and 30 % of the untreated control respectively. &lt;strong&gt;Conclusion: &lt;/strong&gt;&lt;em&gt;P. niruri &lt;/em&gt;inhibited both the entry and HBV replication, thus &lt;em&gt;P. niruri &lt;/em&gt;is a promising candidate for anti-HBV drug development.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">1108</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Tutik Sri Wahyuni&lt;sup&gt;1,2,&lt;/sup&gt;*, Adita Ayu Permanasari&lt;sup&gt;2&lt;/sup&gt;, Aty Widyawaruyanti&lt;sup&gt;1,2,&lt;/sup&gt; Hak Hotta&lt;sup&gt;3,4&lt;/sup&gt;, Chie Aoki-Utsubo&lt;sup&gt;4&lt;/sup&gt;, Achmad Fuad Hafid&lt;sup&gt;1,2 &lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Universitas Airlangga, Jl. Mulyorejo, Surabaya 60115, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Institute of Tropical Disease, Universitas Airlangga, Jl. Mulyorejo, Surabaya 60115, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Faculty of Clinical Nutrition and Dietetics, Konan Women’s University, 6-2-23 Morikitamachi, Higashinada-ku, Kobe 658-0001, JAPAN.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Department of Public Health, Kobe University Graduate School of Health Sciences, 7-10-2, Tomogaoka, Suma-ku, Kobe 654-0142, JAPAN.&lt;/p&gt;
</style></auth-address></record></records></xml>