<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sunita Shailajan</style></author><author><style face="normal" font="default" size="100%">Sasikumar Menon</style></author><author><style face="normal" font="default" size="100%">Dipti Singh</style></author><author><style face="normal" font="default" size="100%">Gauri Swar</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Validated analytical RP-HPLC method for quantitation of wedelolactone from Eclipta alba and marketed Ayurvedic formulations</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Eclipta alba</style></keyword><keyword><style  face="normal" font="default" size="100%">Formulations</style></keyword><keyword><style  face="normal" font="default" size="100%">RP-HPLC</style></keyword><keyword><style  face="normal" font="default" size="100%">Validation</style></keyword><keyword><style  face="normal" font="default" size="100%">Wedelolactone.</style></keyword><keyword><style  face="normal" font="default" size="100%">Whole plant</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2016</style></year><pub-dates><date><style  face="normal" font="default" size="100%">December 2015</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">8</style></volume><pages><style face="normal" font="default" size="100%">132-139</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align:justify&quot;&gt;&lt;strong&gt;Introduction&lt;/strong&gt;: &lt;em&gt;Eclipta alba&lt;/em&gt; Linn. (Asteraceae) is an important ingredient of several Ayurvedic formulations. The monograph on different parts of plant like flowers, leaves, roots are listed in Ayurvedic pharmacopeia of India. The plant is reported to be effective for broad range maladies like inflammation, reproductive problems of females etc. It is also used as a hepatoprotectant, analgesic, antibacterial and antidiabetic agent. Wedelolactone is used as a bioactive marker to establish the quality of the crude drug and its formulations. In the present study, wedelolactone-based standardization of &lt;em&gt;Eclipta alba&lt;/em&gt; and its quantitation from marketed herbal and Ayurvedic formulations has been documented using RP-HPLC. &lt;strong&gt;Methods&lt;/strong&gt;: In the current work, an isocratic method has been developed and validated to quantitate wedelolactone from whole plant of &lt;em&gt;Eclipta alba&lt;/em&gt;. This method is validated as per ICH guidelines and is used to quantitate the content of wedelolactone in polyherbal formulations like Liv52, Geriforte, Mahabhringaraj oil etc. &lt;strong&gt;Results: &lt;/strong&gt;The LOD is found to be 0.5 &amp;mu;g/mL and the LOQ is 1 &amp;mu;g/mL. The linearity range of response is from 5 &amp;mu;g/mL to 100 &amp;mu;g/mL. &lt;strong&gt;Conclusion:&lt;/strong&gt; The validated method is found to be simple, sensitive, accurate, rugged and reproducible. This developed method can be recommended for quality assurance and marker-based standardization of polyherbal formulations containing whole plant of &lt;em&gt;Eclipta alba.&lt;/em&gt;&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">132</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Sunita Shailajan&lt;sup&gt;1&lt;/sup&gt;, Sasikumar Menon&lt;sup&gt;2&lt;/sup&gt;, Dipti Singh&lt;sup&gt;1&lt;/sup&gt;, Gauri Swar&lt;sup&gt;1&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Herbal Research Lab, Ramnarain Ruia College, Matunga, Mumbai - 400 019, India&lt;/p&gt;

&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Institute for Advanced Training and Research in Interdisciplinary Science, Plot No. 194, Scheme No. 6, Road No. 15, Sion Koliwada, Sion (East), Mumbai-400 022, INDIA.&lt;/p&gt;
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