<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Haroon Khan</style></author><author><style face="normal" font="default" size="100%">Murad Ali Khan</style></author><author><style face="normal" font="default" size="100%">Abdul Rauf</style></author><author><style face="normal" font="default" size="100%">Ashhad Haleemi</style></author><author><style face="normal" font="default" size="100%">Shivkanya Fuloria</style></author><author><style face="normal" font="default" size="100%">Neeraj Kumar Fuloria</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Inhibition on Urease and Thermal Induced Protein Denaturation of commonly used Antiulcer Herbal Products. Study based on in-vitro assays</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Akseer ULCER</style></keyword><keyword><style  face="normal" font="default" size="100%">Antiurease activity</style></keyword><keyword><style  face="normal" font="default" size="100%">Endemali</style></keyword><keyword><style  face="normal" font="default" size="100%">Thermal induced protein denaturation.</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2015</style></year><pub-dates><date><style  face="normal" font="default" size="100%">16th Jan, 2015</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">7</style></volume><pages><style face="normal" font="default" size="100%">147-151</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align:justify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;&lt;em&gt;In-vitro&lt;/em&gt; urease inhibitory and thermal induced protein denaturation inhibitory activitieswasperformed for two commonly used herbal productsEndemali and Akseer ULCER in the treatment of ulcers.&lt;strong&gt;Objectives: &lt;/strong&gt;to evaluate the antiulcer potential of two commonly used herbal products, Endemali, Akseer ULCER. &lt;strong&gt;Material and Method:&lt;/strong&gt; In urease inhibitory assay , enzyme solution, extract, diferentregaents added and absorbance was measured at 630 nm (50 min, pH 8.2) and thiourea used as standard. In protein denaturation assay the egg albumin was mixed with different concentration of test compounds, buffer absorbance was measured. Aspirin was used as standard. &lt;strong&gt;Results:&lt;/strong&gt; The Endemalihad a profound effect on the urease activity in a concentration dependent manner with EC&lt;sub&gt;50&lt;/sub&gt; valueof 0.468 mg/ml. The Akseer ULCER antagonized the urease activity markedly with EC&lt;sub&gt;50 &lt;/sub&gt;value of 0.374 mg/ml.These tested herbal products caused marked inhibition of thermal induced protein denaturation in a concentration dependent manner. The potency in the form of EC&lt;sub&gt;50 &lt;/sub&gt;for Endemali, Akseer ULCER was measured as 323, 337 &amp;micro;g/mlrespectively. &lt;strong&gt;Conclusion:&lt;/strong&gt; In short, the tested herbal drug showed strong inhibition on urease activity and inhibition on thermal induced protein denaturation thus our study validated their uses in the treatment of ulcers.&lt;/p&gt;&lt;p style=&quot;text-align:justify&quot;&gt;&lt;strong&gt;Key words:&lt;/strong&gt; Akseer ULCER, Endemali, Antiurease activity, Thermal induced protein denaturation.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">147</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Haroon Khan&lt;sup&gt;*1&lt;/sup&gt;, Murad Ali Khan&lt;sup&gt;2&lt;/sup&gt;, Abdul Rauf&lt;sup&gt;3&lt;/sup&gt;, Ashhad Haleemi&lt;sup&gt;4&lt;/sup&gt;, Shivkanya Fuloria&lt;sup&gt;5&lt;/sup&gt;, Neeraj Kumar Fuloria&lt;sup&gt;5 &lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Pharmacy, Abdul Wali Khan University Mardan 23200, Pakistan&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Chemistry, Kohat University of Science and Technology, Kohat, Pakistan&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Institute of Chemical Sciences, University of Peshawar, Peshawar -25120, Pakistan&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Department of Pharmacy, University of Peshawar, Peshawar -25120, Pakistan&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;Department of Pharmcy, AIMST University,Semeling Campus, Bedong, Kedah Darul Aman-08100, Malyasia&lt;/p&gt;</style></auth-address></record></records></xml>