<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Amira Abdel Motaal</style></author><author><style face="normal" font="default" size="100%">Shahira M. Ezzat</style></author><author><style face="normal" font="default" size="100%">Hesham El-Askary</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Antihyperglycemic Activity and Standardization of the Bioactive Extract of Cleome droserifolia Growing in Egypt</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">7-dirhamnoside</style></keyword><keyword><style  face="normal" font="default" size="100%">Antihyperglycemic</style></keyword><keyword><style  face="normal" font="default" size="100%">Cleome droserifolia</style></keyword><keyword><style  face="normal" font="default" size="100%">high performance liquid chromatography standardization</style></keyword><keyword><style  face="normal" font="default" size="100%">kaempferol-4’-methoxy-3</style></keyword><keyword><style  face="normal" font="default" size="100%">Validation</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2nd July 2014</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">6</style></volume><pages><style face="normal" font="default" size="100%">15-21</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Background:&lt;/strong&gt;&lt;em&gt;Cleome droserifolia&lt;/em&gt; herb is well known in the Egyptian folk medicine for the treatment of diabetes. However, a standardized active extract of the herb was never prepared for incorporation into a pharmaceutical dosage form. &lt;strong&gt;Materials and Methods:&lt;/strong&gt; Comparative high performance liquid chromatography (HPLC) chromatographic profi les were established in order to study the ethnopharmacological use of the antihyperglycemic herb using a validated reversed phase- HPLC method which was developed for standardization of the active aqueous extract. A biologically guided fractionation of the antihyperglycemic aqueous extract was carried out &lt;em&gt;in vivo&lt;/em&gt; using alloxan induced diabetic rats. &lt;strong&gt;Results:&lt;/strong&gt; The aqueous extract contained the highest percent of the total active fl avonol glycosides (78.20%) compared to the 70% and 50% ethanolic extracts (51.17 and 42.66%, respectively). The aqueous extract and its ethyl acetate fraction possessed the highest antihyperglycemic activities. A standard calibration curve, established for the major bioactive methoxylated fl avonol glycoside (kaempferol-4&amp;rsquo;-methoxy-3,7-dirhamnoside) at a concentration range of 44-174 &amp;mu;g/ml, showed good linearity with a correlation coeffi cient (R2) of 0.998. The recovery of the method was 100.5%. A high degree of precision (relative standard deviation values &amp;lt;5%) was achieved. The limits of detection and quantifi cation were 0.01 and 0.02 &amp;mu;g/ml, respectively, indicating the sensitivity of the method. &lt;strong&gt;Conclusion:&lt;/strong&gt; The aqueous extract contained the highest percent of the total active fl avonol glycosides. The extract, standardized to contain not &amp;lt;1.5 &amp;plusmn; 0.06% of kaempferol-4&amp;rsquo;-methoxy- 3,7-dirhamnoside, was tested at three different dose levels showing a 63.3% activity of that of metformin at100 mg/kg body weight. Furthermore, it raised the blood insulin level by 146.26% at this dose level.&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Key words:&lt;/strong&gt; Antihyperglycemic, &lt;em&gt;Cleome droserifolia&lt;/em&gt;, high performance liquid chromatography standardization, kaempferol-4&amp;rsquo;-methoxy-3,7-dirhamnoside, validation.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Amira Abdel Motaal&lt;sup&gt;1,2&lt;/sup&gt;, Shahira M. Ezzat&lt;sup&gt;1*&lt;/sup&gt;, Hesham El-Askary&lt;sup&gt;1,3&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Kasr-El-Ainy St., Cairo, 11562, Egypt,&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Pharmacognosy, Faculty of Pharmacy and Drug Technology, Heliopolis University, El-Horreya 2834, Cairo, Egypt,&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Pharmacognosy, Faculty of Pharmaceutical Sciences and Pharmaceutical Technology, Future University, New Cairo, 11528, Egypt.&lt;/p&gt;</style></auth-address></record></records></xml>