<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Harita Parikh</style></author><author><style face="normal" font="default" size="100%">Aparna Khanna</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Pharmacognosy and Phytochemical Analysis of Brassica juncea Seeds</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antioxidant</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytotoxicity</style></keyword><keyword><style  face="normal" font="default" size="100%">Fourier Transform Infrared Spectroscopy</style></keyword><keyword><style  face="normal" font="default" size="100%">High performance Thin layer Chromatography</style></keyword><keyword><style  face="normal" font="default" size="100%">microscopy</style></keyword><keyword><style  face="normal" font="default" size="100%">Phenolics</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2nd July 2014</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">6</style></volume><pages><style face="normal" font="default" size="100%">47-54</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt;&lt;em&gt;Brassica juncea&lt;/em&gt; is an economically important plant that has been well-known in India for centuries for its medicinal and nutritive values. The broad spectrum of beneficial effects of the seeds perceived with this plant warrants further exploration of &lt;em&gt;B. juncea&lt;/em&gt; seeds as a potential source for obtaining pharmacologically standardized phytotherapeutics, which could be potentially useful. The objective of the present study was to perform the pharmacognosy of mustards seeds inclusive of qualitative and quantitative phytochemical analysis, fingerprinting by infrared spectroscopy and high performance thin layer chromatography analysis and toxicity assessment &lt;em&gt;in vitro&lt;/em&gt;. &lt;strong&gt;Methods: &lt;/strong&gt;Different sections of seeds were taken and stained with 0.1% phloroglucinol for microscopic examination. The seeds were extracted by 80% alcohol on a rotary shaker to perform phytochemical analysis and fingerprinting. The toxicity assessment of this extract was performed on human dermal fibroblast cells. &lt;strong&gt;Results:&lt;/strong&gt; Microscopic examination of seeds showed characteristic features of mustard seeds. The extraction of these seeds by 20% alcohol resulted in IC&lt;sub&gt;50&lt;/sub&gt; value of 103 &amp;plusmn; 3 &amp;mu;g/mL for 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl radical scavenging assay. The fingerprinting analysis of this extract indicated probable presence of sinigrin, quercetin, vanillin, catechin, vitamin E and sulfur-containing compounds. This extract exhibited 50% toxicity (IC&lt;sub&gt;50&lt;/sub&gt;) at 1.79 mg/mL. &lt;strong&gt;Conclusion:&lt;/strong&gt; The result achieved will be used to assess the therapeutic efficacy of seed extracts for future pharmacological evaluations.&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Key words:&lt;/strong&gt; Antioxidant, cytotoxicity, Fourier transform infrared spectroscopy, high performance thin layer chromatography, microscopy, phenolics.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Harita Parikh, Aparna Khanna&lt;sup&gt;*&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;&lt;p style=&quot;text-align: justify;&quot;&gt;Department of Biological Sciences, School of Science, NMIMS University, Vile Parle (West), Mumbai, Maharashtra, India.&lt;/p&gt;</style></auth-address></record></records></xml>