<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Afriwardi</style></author><author><style face="normal" font="default" size="100%">Rahmad Abdillah</style></author><author><style face="normal" font="default" size="100%">Elidahanum Husni</style></author><author><style face="normal" font="default" size="100%">Hafifah Hardini</style></author><author><style face="normal" font="default" size="100%">Khalila Tri Syahbani Zuler</style></author><author><style face="normal" font="default" size="100%">Aditya Alqamal Alianta</style></author><author><style face="normal" font="default" size="100%">Yufri Aldi</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Subacute Toxicity Test of Hydrocotyle Sibthorpioides Lam. Extract on Histopathological Images of Liver and Kidney of White Male Mice</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Histopathology</style></keyword><keyword><style  face="normal" font="default" size="100%">Hydrocotyle sibthorpioides Lam.</style></keyword><keyword><style  face="normal" font="default" size="100%">Kidney</style></keyword><keyword><style  face="normal" font="default" size="100%">LD50</style></keyword><keyword><style  face="normal" font="default" size="100%">Liver</style></keyword><keyword><style  face="normal" font="default" size="100%">Subacute.</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2022</style></year><pub-dates><date><style  face="normal" font="default" size="100%">October 2022</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">14</style></volume><pages><style face="normal" font="default" size="100%">619-626</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Introduction:&lt;/strong&gt;&lt;em&gt; Hydrocotyle sibthorpioides&lt;/em&gt; Lam. in Indonesia known as pegagan embun. It has been used to increase the immune system and has been shown to have immunostimulating, anti-inflammatory and hematopoietic effects. However, there is no scientific evidence that shows this plant is safe for long-term use. Based on that circumstance, this study aimed to measure the safety of Pegagan Embun (&lt;em&gt;Hydrocotyle sibthorpioides&lt;/em&gt; Lam.) ethanol extract activities on liver and kidney histopathology. &lt;strong&gt;Aim:&lt;/strong&gt; The study aimed to measure the safety of Pegagan Embun (&lt;em&gt;Hydrocotyle sibthorpioides &lt;/em&gt;Lam.) ethanol extract activities on liver and kidney histopathology. &lt;strong&gt;Material and Method&lt;/strong&gt;: Ethanol extract used because all the active compounds in plants extracted as a whole, and it cheaper and more efficient in the extraction process. Determine as many thirty-six white male mice as test animals and separate them into eight treatment groups. The administrated ethanol extract of Pegagan Embun (&lt;em&gt;Hydrocotyle sibthorpioides&lt;/em&gt; Lam.) at doses of 7, 35, and 150 mg/kg BW for 7, 14, and 21 days. On days 8&lt;sup&gt;th&lt;/sup&gt;, 15&lt;sup&gt;th&lt;/sup&gt;, and 22&lt;sup&gt;nd&lt;/sup&gt;, three white male mice collected from each treatment group and collected their liver and kidney. The data analysed used a T-test with IBM SPSS type 24. &lt;strong&gt;Result: &lt;/strong&gt;LD50 of ethanol extract of &lt;em&gt;Hydrocotyle sibthorpioides&lt;/em&gt; Lam. &amp;gt; 15,000 mg/kg means practically not toxic. The results showed that the administration of extract &lt;em&gt;Hydrocotyle sibthorpioides &lt;/em&gt;Lam. for 7, 14, and 21 days showed a non-significant effect on any histological damage to the liver of male white mice at doses of 7 and 35 mg/kg BW (normal histology). The non-significant effect also occurs at150 mg/kg BW for 7 days; however, it caused mild damage at a dose of 150 mg/kg BW for 14 days and moderate damage at 150 mg/kg BW for 21 days. In renal histopathology, doses of 7 mg/kg BW. for 7, 14, and 21 days showed normal histology and doses of 35 mg/kg BW for 7, 14, and 21 days showed minimal damage. The administration at doses of 150 mg/kg BW for 7 days showed mild damage, while a dose of 150 mg/kg BW for 14 and 21 days showed moderate damage. &lt;strong&gt;Conclusion:&lt;/strong&gt; It concluded that the administration of extract of &lt;em&gt;Hydrocotyle sibthorpioides&lt;/em&gt; Lam. did not cause severe damage to the histology of the liver and kidneys of white male mice.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">619</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Afriwardi&lt;sup&gt;1&lt;/sup&gt;, Rahmad Abdillah&lt;sup&gt;2&lt;/sup&gt;, Elidahanum Husni&lt;sup&gt;3&lt;/sup&gt;, Hafifah Hardini&lt;sup&gt;4&lt;/sup&gt;, Khalila Tri Syahbani Zuler&lt;sup&gt;4&lt;/sup&gt;, Aditya Alqamal Alianta&lt;sup&gt;5&lt;/sup&gt;, Yufri Aldi&lt;sup&gt;6,*&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;lecturer in the Department of Physiology also as the Dean of Faculty of Medicine Universitas Andalas, Dean at the Faculty of Dentistry of Universitas Andalas, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;lecturer in Department of Pharmacology, Faculty of Pharmacy, Universitas Andalas, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;lecturer in Department of Biology of Pharmacy, Faculty of Pharmacy, Universitas Andalas, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Profession student in Pharmacist Program at Faculty of Pharmacy, Universitas Andalas, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;lecturer in Department on Socio-economic Animal Science, Faculty of Animal Science, Universitas Andalas, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;6&lt;/sup&gt;lecturer in Department of Pharmacology, Faculty of Pharmacy, Universitas Andalas, INDONESIA&lt;/p&gt;
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