<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Wirda Anggraini</style></author><author><style face="normal" font="default" size="100%">Djoko Agus Purwanto</style></author><author><style face="normal" font="default" size="100%">Idha Kusumawati</style></author><author><style face="normal" font="default" size="100%">Isnaeni3, Suryanto</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Influence of the Environment on Biofilm Formation Candida albicans of Vulvovaginal Candidiasis Isolate Patient</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Biofilm formation; Candida albicans; sabourau dextrose broth; potato dextrose broth; vulvovaginal candidiasis</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2023</style></year><pub-dates><date><style  face="normal" font="default" size="100%">March 2023</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">15</style></volume><pages><style face="normal" font="default" size="100%">216-222</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Context: &lt;/strong&gt;Candida albicans is a type of fungus that can produce biofilms and may cause Vulvovaginal Candidiasis (VVC) disease. We investigated the effect of environment on biofilm formation of &lt;em&gt;C. albicans&lt;/em&gt; patient isolates and ATCC 14053. Biofilm formation is influenced by several factors such as environments and nutrients. Objectives: To investigated the effect of environment on biofilm formation of &lt;em&gt;C. albicans&lt;/em&gt; patient isolates and ATCC 14053. &lt;strong&gt;Methods:&lt;/strong&gt; The samples using &lt;em&gt;C. albican&lt;/em&gt;s ATCC 14053,&lt;em&gt; C. albicans,&lt;/em&gt; which may form biofilms, was isolated from patient Dermatology and Venereology and Obstetrics and Gynecology from a hospital in Malang. TCP (A tissue Culture Plate) is the biofilm formation method used. &lt;strong&gt;Results: &lt;/strong&gt;Biofilm formation took 48-72 hours at 25 °C and 96-120 hours at 37 °C. Based on the result biofilm formation of &lt;em&gt;C. albicans&lt;/em&gt; is influenced by environmental factors and characterized by a high OD value. &lt;strong&gt;Conclusions:&lt;/strong&gt; Biofilm formation is accelerated in temperature incubation needed at 25 °C for 48-72 hours, using biomass 107 CFU/mL, nutrition using Potato Dextrose Broth media and 1% glucose, and the solvent of 30% acetic acid to obtain acid condition.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">216</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Wirda Anggraini&lt;sup&gt;1,2&lt;/sup&gt;, Djoko Agus Purwanto&lt;sup&gt;1*&lt;/sup&gt;, Idha Kusumawati&lt;sup&gt;1&lt;/sup&gt;, Isnaeni&lt;sup&gt;3&lt;/sup&gt;, Suryanto&lt;sup&gt;4&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Doctor of Science Pharmacy, Faculty Pharmacy, Airlangga University, Surabaya, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Pharmacy, Faculty Medicine and Health Sciences, Maulana Malik Ibrahim State Islamic University Malang, Malang, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Pharmacy, Faculty Health Science, Muhammadiyah University Surabaya, Surabaya, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Master Student of Science Pharmacy, Faculty of Pharmacy, Airlangga University, Surabaya, INDONESIA.&lt;/p&gt;
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