<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Jesús Rojas-Jaimes</style></author><author><style face="normal" font="default" size="100%">Luis Castañeda Pelaez</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Generation of Multinucleated Giant Cells due to Leishmania (V.) braziliensis Infection</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Immunity</style></keyword><keyword><style  face="normal" font="default" size="100%">Infection</style></keyword><keyword><style  face="normal" font="default" size="100%">Leishmania (V.) braziliensis</style></keyword><keyword><style  face="normal" font="default" size="100%">Macrophage</style></keyword><keyword><style  face="normal" font="default" size="100%">Multinucleated Giant Cells</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2023</style></year><pub-dates><date><style  face="normal" font="default" size="100%">October 2023</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">15</style></volume><pages><style face="normal" font="default" size="100%">905-909</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background&lt;/strong&gt;: Multinucleated giant cells were first described by Langhans(MGCs), these MGCs have a role in innate immunity that includes extracellular matrix remodeling associated with granuloma formation; another function of them is to participate in the removal of cellular debris in apoptosis during certain infections. &lt;strong&gt;Objective: &lt;/strong&gt;Generate multinucleated giant cells due to &lt;em&gt;Leishmania &lt;/em&gt;(V.) braziliensis infection. &lt;strong&gt;Methods:&lt;/strong&gt; For infection macrophage/parasite ratio of 1 to 10 using the RAW 264.7-line (5 x10&lt;sup&gt;5&lt;/sup&gt;/mL) and &lt;em&gt;Leishmania &lt;/em&gt;(V.) &lt;em&gt;braziliensis &lt;/em&gt;metacyclic promastigotes (50 x105/mL) was a strain maintained in culture Schneider liquid medium, supplemented with 20% fetal bovine serum and 10 000 U/10 mg/mL penicillin streptomycin at pH 7 and 25 °C in the laboratory. Incubation was continued for 4 days, and microscopic observation at 1000X was performed at 24 hours and 96 hours, respectively. &lt;strong&gt;Results: &lt;/strong&gt;In our study an MGC conversion was observed at 96 hours (50% ± 28.2). &lt;strong&gt;Conclusions:&lt;/strong&gt; In vitro MGC model could be used to study the physiopathology of MGC generation by &lt;em&gt;Leishmania &lt;/em&gt;(V.) &lt;em&gt;braziliensis &lt;/em&gt;infection.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><work-type><style face="normal" font="default" size="100%">Research Article</style></work-type><section><style face="normal" font="default" size="100%">905</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p&gt;&lt;strong&gt;Jesús Rojas-Jaimes&lt;sup&gt;1,2,*&lt;/sup&gt;, Luis Castañeda Pelaez&lt;sup&gt;1&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;1&lt;/sup&gt;Facultad de Ciencias de la Salud, Universidad Privada del Norte, Lima, PERÚ.&lt;/p&gt;

&lt;p&gt;&lt;sup&gt;2&lt;/sup&gt;Escuela de Medicina Humana, Universidad Científica del Sur, Lima, PERÚ.&lt;/p&gt;
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