<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Zuraida</style></author><author><style face="normal" font="default" size="100%">Silvia Werdhy Lestari</style></author><author><style face="normal" font="default" size="100%">Mulyoto Pangestu</style></author><author><style face="normal" font="default" size="100%">Andon Hestiantoro</style></author><author><style face="normal" font="default" size="100%">Kusmardi Kusmardi</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Effect of Glutathione Supplementation in Cryoprotectant Modification on Tyrosine Phosphorylation, Acrosin Expression and Acrosome Reaction of Post-Thawing Spermatozoa Quality</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">acrosin expression</style></keyword><keyword><style  face="normal" font="default" size="100%">acrosome reaction</style></keyword><keyword><style  face="normal" font="default" size="100%">CPA modification</style></keyword><keyword><style  face="normal" font="default" size="100%">glutathione (GSH)</style></keyword><keyword><style  face="normal" font="default" size="100%">spermatozoa cryopreservation</style></keyword><keyword><style  face="normal" font="default" size="100%">tyrosine phosphorylation</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2024</style></year><pub-dates><date><style  face="normal" font="default" size="100%">June 2024</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">16</style></volume><pages><style face="normal" font="default" size="100%">554-562</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background:&lt;/strong&gt; Tyrosine phosphorylation, acrosin, and acrosome reaction play an important role in fertilisation. However, cryopreservation causes changes in tyrosine phosphorylation, acrosin expression, and acrosome reaction which affect the quality of spermatozoa. Cryoprotectant media added with antioxidants is needed to protect Spermatozoa from the effects of cryopreservation so that the quality of spermatozoa can be maintained. &lt;strong&gt;Objectives:&lt;/strong&gt; This research examined the effect of glutathione (GSH) supplementation in cryopreservation media on tyrosine phosphorylation, acrosin expression, and acrosome reaction. In this research, pure modified Cryoprotectant (CPA) was compared with CPA supplemented with GSH in three different concentrations. &lt;strong&gt;Materials and Methods:&lt;/strong&gt; The research sample was male mus musculus albinus strain Deutchland Denken Yoken (DDY). Mice spermatozoa was cryopreserved and several parameters were measured including tyrosine phosphorylation, acrosin expression, and acrosome reaction. &lt;strong&gt;Results:&lt;/strong&gt; The addition of GSH to the modified CPA increased tyrosine phosphorylation, acrosin expression, and acrosome reaction (maintaining acrosome integrity). The group with 1.00 mM GSH obtained the highest results among the other groups. Significant increases were found in tyrosine phosphorylation, acrosin expression, and acrosome reaction after the addition of 1.00 mM GSH. &lt;strong&gt;Conclusion:&lt;/strong&gt; Glutathione supplementation in modified CPA can increase tyrosine phosphorylation, acrosin expression, and acrosome reaction of frozen-thawed spermatozoa. Treatment using GSH at a dose of 1.00 mM is the most effective and modification of CPA with the addition of glutathione can improve the tyrosine phosphorylation, acrosin expression and acrosome reaction in cryopreserved spermatozoa.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">554</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Zuraida&lt;sup&gt;1,2&lt;/sup&gt;, Silvia Werdhy Lestari&lt;sup&gt;3&lt;/sup&gt;,*, Mulyoto Pangestu&lt;sup&gt;4&lt;/sup&gt;, Andon Hestiantoro&lt;sup&gt;5&lt;/sup&gt;, Kusmardi Kusmardi&lt;sup&gt;6-8&lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Doctoral Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia; Jl. Salemba Raya No. 6, Jakarta 10430 INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Midwifery, Faculty of Health, Universitas Fort de Kock Bukittinggi, Jl. Soekarno Hatta No. 11, Bukittinggi, Sumbar 26117 INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Medical Biology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430 INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Education Program in Reproduction and Development, Department of Obstetrics and Gynecology, School of Clinical Sciences, Monash University, Victoria, AUSTRALIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430 INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;6&lt;/sup&gt;Department of Pathological Anatomy, Faculty of Medicine, Universitas Indonesia, Jalan Salemba Raya No. 6, Jakarta 10430, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;7&lt;/sup&gt;Drug Development Research Center, Indonesia Medical Education and Research Institute (IMERI), Universitas Indonesia, Jalan Salemba Raya No. 6, Jakarta 10430, INDONESIA.&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;8&lt;/sup&gt;Human Cancer Research Center, Indonesia Medical Education and Research Institute (IMERI), Universitas Indonesia, Jalan Salemba Raya No. 6, Jakarta 10430, INDONESIA.&lt;/p&gt;
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