<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Tiwuk Susantiningsih</style></author><author><style face="normal" font="default" size="100%">Ichwan Baihaki</style></author><author><style face="normal" font="default" size="100%">Maria Selvester Thadeaus</style></author><author><style face="normal" font="default" size="100%">Yuni Setyaningsih</style></author><author><style face="normal" font="default" size="100%">Mila Citrawati</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">In-Silico Study of Bioactive Compounds from Moringa oleifera Fruit as Anti Premature Senescence Agents in Cardiac Cells: A Study on the p53 Protein</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antioxidant</style></keyword><keyword><style  face="normal" font="default" size="100%">cellular senescence</style></keyword><keyword><style  face="normal" font="default" size="100%">in silico</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular docking</style></keyword><keyword><style  face="normal" font="default" size="100%">Moringa oleifera</style></keyword><keyword><style  face="normal" font="default" size="100%">p53 protein</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2025</style></year><pub-dates><date><style  face="normal" font="default" size="100%">August 2025</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">17</style></volume><pages><style face="normal" font="default" size="100%">497-505</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Background: &lt;/strong&gt;Cellular senescence, characterized by irreversible cell cycle arrest, contributes significantly to the pathogenesis of cardiovascular diseases through mechanisms involving oxidative stress and activation of p53-mediated signaling. &lt;em&gt;Moringa oleifera&lt;/em&gt;, widely recognized for its antioxidant properties, has demonstrated anti-aging effects; however, the specific bioactive compounds within its fruit and their mechanisms of action remain poorly understood. &lt;strong&gt;Objective: &lt;/strong&gt;This study aimed to investigate the potential of &lt;em&gt;M. oleifera &lt;/em&gt;fruit-derived compounds as anti-premature senescence agents targeting the p53 protein using in-silico molecular docking approaches. Methods: Bioactive compounds from &lt;em&gt;M. oleifera&lt;/em&gt; fruit were screened via molecular docking against the human p53 protein, with Nutlin-3 used as a positive control. Binding affinities, hydrogen bonding, and hydrophobic interactions were analyzed to determine ligand– receptor interactions. &lt;strong&gt;Results: &lt;/strong&gt;Niacin and oxalic acid exhibited stronger binding affinities (–5.90 and –6.00 kcal/mol, respectively) compared to Nutlin-3 (–5.64 kcal/mol). Niacin formed stable hydrogen bonds and hydrophobic interactions with key residues within the p53 active site, suggesting a capacity to modulate p53 activity. Oxalic acid demonstrated the highest binding affinity but lacked hydrogen bonding, indicating potential instability despite strong interaction. These findings support previous studies highlighting &lt;em&gt;M. oleifera's &lt;/em&gt;role in ROS suppression and p53 modulation, pointing to its therapeutic relevance in mitigating cellular aging. &lt;strong&gt;Conclusion: &lt;/strong&gt;Niacin and oxalic acid from &lt;em&gt;M. oleifera&lt;/em&gt; exhibit promising binding characteristics as modulators of the p53 pathway. Their anti-senescence potential warrants further validation through molecular dynamics simulations and biological assays. This study supports the development of natural compound-based therapeutics for age-related cardiac degeneration.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">497</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p class=&quot;rtejustify&quot;&gt;&lt;strong&gt;Tiwuk Susantiningsih&lt;sup&gt;1,2&lt;/sup&gt;, Ichwan Baihaki&lt;sup&gt;1*&lt;/sup&gt;, Maria Selvester Thadeaus&lt;sup&gt;1,2&lt;/sup&gt;, Yuni Setyaningsih&lt;sup&gt;1,2&lt;/sup&gt;, Mila Citrawati&lt;sup&gt;1,2&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Faculty of Medicine, Universitas Pembangunan Nasional Veteran Jakarta, South Jakarta, Jakarta, 12450, INDONESIA&lt;/p&gt;

&lt;p class=&quot;rtejustify&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Research Centre for Moringa Oleifera, Universitas Pembangunan Nasional Veteran Jakarta, South Jakarta, Jakarta, 12450, INDONESIA&lt;/p&gt;
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