<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sri Atun</style></author><author><style face="normal" font="default" size="100%">Sri Handayani</style></author><author><style face="normal" font="default" size="100%">Anna Rakhmawati</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Potential Bioactive Compounds Isolated from Boesenbergia rotunda as Antioxidant and Antimicrobial Agents</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antimicrobial</style></keyword><keyword><style  face="normal" font="default" size="100%">Antioxidant</style></keyword><keyword><style  face="normal" font="default" size="100%">Bioactive compound</style></keyword><keyword><style  face="normal" font="default" size="100%">Boesenbergia rotunda</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">March 2018</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://fulltxt.org/article/517</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">513-518</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Objective:&lt;/strong&gt; This study was conducted to identify some bioactive compounds of &lt;em&gt;Boesenbergia rotunda rhizome&lt;/em&gt; and to test as antioxidant and antimicrobial agents. &lt;strong&gt;Methods:&lt;/strong&gt; The milled dried rhizome of &lt;em&gt;B. Rotunda&lt;/em&gt; (5kg) was extracted exhaustively with ethanol. The ethanol extract was partitioned three times by n-hexane, chloroform, and ethyl acetate respectively. Each fraction was fractionated by vacuum liquid chromatography (VLC) and then purified by column chromatography gravitation. Structural identification of all pure compounds were elucidated based on spectroscopic methods (UV, IR, and NMR).The antioxidant activity was tested by 2,2-diphenyl- 1-picrylhydrazyl (DPPH). Antimicrobial character was screened for activities againts pathogenic bacteria i.e. &lt;em&gt;Escherichia coli&lt;/em&gt; ATCC-11229, Staphylococcus aureus ATCC-25923, Staphylococcus epidermidis FNCC-0048, and Streptococcus mutans by the disk-diffusion method. The assay was done in triplicate, and chloramphenicol was used as the positive control. Results and &lt;strong&gt;Discussion:&lt;/strong&gt; From ethanol extract of &lt;em&gt;B. rotunda&lt;/em&gt; three known compounds of flavanones, namely 2&amp;rsquo;,4&amp;rsquo;-dihydroxy-6-methoxychalcone (compound-1), 5-hydroxy-7- methoxyflavanone (compound-2), and 5,7-dihydroxyflavanone (compound-3) were isolated. The ethanol extract of &lt;em&gt;B. rotunda&lt;/em&gt; and the three isolated compounds -1,-2, and -3 showed an antioxidant activity with the IC value of 92.64; 46.66; 62.84, and 62.66 &amp;mu;g/mL, respectively. The zone of inhibition of extract and the three isolated compounds showed moderate activity against &lt;em&gt;Escherichia coli&lt;/em&gt; ATCC-11229, &lt;em&gt;Staphylococcus aureus&lt;/em&gt; ATCC-25923, &lt;em&gt;Staphylococcus epidermidis&lt;/em&gt; FNCC-0048, and &lt;em&gt;Streptococcus mutans&lt;/em&gt;.The maximum zone of inhibition was 13.20&amp;plusmn;0.76 mm at the maximum concentration used (500 &amp;mu;g/mL) against &lt;em&gt;Escherichia coli&lt;/em&gt; ATCC-11229, and the minimum inhibitory concentration(MIC) for each bacteria was found to be 0.5&amp;mu;g/mL. &lt;strong&gt;Conclusion:&lt;/strong&gt; The result of the study suggests that &lt;em&gt;B. rotunda&lt;/em&gt; rhizome contains potential bioactive compounds which could be suitable for antioxidant and the treatment of various infections caused by &lt;em&gt;Escherichia coli&lt;/em&gt; ATCC-11229, &lt;em&gt;Staphylococcus aureus&lt;/em&gt; ATCC- 25923, &lt;em&gt;Staphylococcus epidermidis&lt;/em&gt; FNCC-0048, and &lt;em&gt;Streptococcus mutans&lt;/em&gt;.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">513</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Sri Atun&lt;sup&gt;1*&lt;/sup&gt;, Sri Handayani&lt;sup&gt;1&lt;/sup&gt;, Anna Rakhmawati&lt;sup&gt;2 &lt;/sup&gt;&lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Chemistry Education, Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta, INDONESIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Biology Education, Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta, INDONESIA.&lt;/p&gt;</style></auth-address></record></records></xml>