<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ashwani Kumar Bhardwaj</style></author><author><style face="normal" font="default" size="100%">Avilekh Naryal</style></author><author><style face="normal" font="default" size="100%">Pushpender Bhardwaj</style></author><author><style face="normal" font="default" size="100%">Ashish Rambhau Warghat</style></author><author><style face="normal" font="default" size="100%">Balpreet Arora</style></author><author><style face="normal" font="default" size="100%">Shikha Dhiman</style></author><author><style face="normal" font="default" size="100%">Shweta Saxena</style></author><author><style face="normal" font="default" size="100%">Pratap Kumar Pati</style></author><author><style face="normal" font="default" size="100%">Om Prakash Chaurasia</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">High Efficiency in vitro Plant Regeneration and Secondary Metabolite Quantification from Leaf Explants of Rhodiola imbricata</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Acclimatization</style></keyword><keyword><style  face="normal" font="default" size="100%">Cinnamyl alcohol</style></keyword><keyword><style  face="normal" font="default" size="100%">Endangered</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant growth regulator</style></keyword><keyword><style  face="normal" font="default" size="100%">Regeneration system</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">March 2018</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://fulltxt.org/article/510</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">470-475</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Aim:&lt;/strong&gt; &lt;em&gt;Rhodiola imbricata&lt;/em&gt; is an endangered medicinal plant of the trans-Himalayan Leh-Ladakh region belonging to the family Crassulaceae. An efficient propagation and regeneration system via direct shoot organogenesis from leaf explant and evaluation of cinnamyl alcohol (Secondary metabolite) was established in this study. &lt;strong&gt;Material and Methods:&lt;/strong&gt; &lt;em&gt;In vitro&lt;/em&gt; grown leaves were inoculated using Murashige and Skoog (MS) medium supplemented with (alpha)- naphtalene acetic acid (NAA) in combination with 6- benzyladenine (BAP) for callus proliferation and regeneration. &lt;strong&gt;Results:&lt;/strong&gt; The highest percentage of rhizogenous callus was induced in medium containing NAA (10.0-15.0 &lt;em&gt;&amp;mu;&lt;/em&gt;M). The highest percentage of shoot formation from leaf derived callus was obtained in the medium containing of NAA (5.0 &lt;em&gt;&amp;mu;&lt;/em&gt;M) and BAP (2.5 &lt;em&gt;&amp;mu;&lt;/em&gt;M) as well as in NAA (1.0 &amp;mu;M), BAP (5.0 &lt;em&gt;&amp;mu;&lt;/em&gt;M) (38.88% and 37.49%) respectively. Rooting of regenerated shoots were effective when a lower concentration of NAA (0.5 &lt;em&gt;&amp;mu;&lt;/em&gt;M) was used alone. A maximum number of roots (22.0) and higher length (0.6 cm) was observed. The &lt;em&gt;in-vitro&lt;/em&gt; plantlets with well-developed shoots and roots were acclimatized successfully to natural field conditions with a survival rate of over 80%. Cinnamyl alcohol (Secondary metabolite) evaluation was also done for the very first time and an upregulation of 49.6% and 30.6% were observed in in-vitro roots and shoots when compared with mother root and shoot respectively. Hence, it was proved that the content of secondary metabolites obtained from &lt;em&gt;in-vitro&lt;/em&gt; raised plants is higher than mother plant. &lt;strong&gt;Conclusion:&lt;/strong&gt; These results may lay a foundation for genetic improvement and can be used to determine sequential level of targeted secondary metabolites through cell culture in &lt;em&gt;Rhodiola imbricata&lt;/em&gt;.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">470</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Ashwani Kumar Bhardwaj&lt;sup&gt;1&lt;/sup&gt;, Avilekh Naryal&lt;sup&gt;1&lt;/sup&gt;, Pushpender Bhardwaj&lt;sup&gt;1&lt;/sup&gt;, Ashish Rambhau Warghat&lt;sup&gt;1,3&lt;/sup&gt;, Balpreet Arora&lt;sup&gt;2&lt;/sup&gt;, Shikha Dhiman&lt;sup&gt;1&lt;/sup&gt;, Shweta Saxena&lt;sup&gt;1&lt;/sup&gt;, Pratap Kumar Pati&lt;sup&gt;2&lt;/sup&gt;, Om Prakash Chaurasia&lt;sup&gt;1*&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Biotechnology, Defence Institute of High Altitude Research, Defence Research and Development Organization, Leh-194101, INDIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Department of Biotechnology, Guru Nank Dev University, Amritsar-143040, Punjab, INDIA.&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Biotechnology, Institute of Himalayan Bioresource Technology, Council of Scientific and Industrial Research, Palampur-176061, Himachal Pradesh, INDIA.&lt;/p&gt;</style></auth-address></record></records></xml>