<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Robert Hotman Sirait</style></author><author><style face="normal" font="default" size="100%">Mochammad Hatta</style></author><author><style face="normal" font="default" size="100%">Syafri K.Arief</style></author><author><style face="normal" font="default" size="100%">Tigor P. Simanjuntak</style></author><author><style face="normal" font="default" size="100%">Bambang Suprayogi</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Profile of HMGB1 mRNA Expression and TLR4 Protein in BALB/c Mice Model Sterile Injury after Systemic Lidocaine Administration</style></title><secondary-title><style face="normal" font="default" size="100%">Pharmacognosy Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">HMGB1 mRNA</style></keyword><keyword><style  face="normal" font="default" size="100%">lidocaine</style></keyword><keyword><style  face="normal" font="default" size="100%">Sterile injury</style></keyword><keyword><style  face="normal" font="default" size="100%">TLR4</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">March 2018</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://fulltxt.org/article/529</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">586-589</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Background:&lt;/strong&gt; High mobility group box 1 (HMGB1) is a cytokine proinflamation which contributes to inflammation. HMGB1 physically interacts with toll like receptor 4 (TLR4) to release macrophage cytokines. The aim of this study was to demonstrate the effectiveness of systemic lidocaine administration to inhibit the expression of HMGB1 mRNA and TLR4 protein in mice BALB/c mice with sterile injury. &lt;strong&gt;Material and Methods:&lt;/strong&gt; Twenty adult male BALB/c mice were divided into lidocaine and control groups. A sterile injury is done by closed fracturing the left thigh bone of the mice. The lidocaine group was treated with 2 mg/kgBW lidocaine through tail vein injection after 4 h of sterile injury. The control group was given distilled water therapy as a substitute for lidocaine. Mice blood is extracted from the tail vein before trauma, 4 h after trauma, and 2 h after the administration of lidocaine and distilled water is complete. The HMGB1 mRNA expression was examined by quantitative real-time polymerase chain reaction (qPCR) while the TLR4 protein level was determined with enzyme-linked immunosorbent assay (ELISA) according to the manufacturer&amp;rsquo;s instructions. &lt;strong&gt;Result:&lt;/strong&gt; The HMGB1 mRNA expression and TLR4 protein levels in BALB/c that sustained inflammation due to a sterile injury was significantly decreased in the lidocaine group (&lt;em&gt;p&lt;/em&gt; &amp;lt; 0.00). &lt;strong&gt;Conclusion:&lt;/strong&gt; Administration systemic 2 mg/kgBW of lidocaine is effectively inhibits HMGB1 mRNA and TLR4 protein in mice that sustain inflammation due to a sterile injury.&amp;nbsp;&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Original Article</style></work-type><section><style face="normal" font="default" size="100%">586</style></section><auth-address><style face="normal" font="default" size="100%">&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;strong&gt;Robert Hotman Sirait&lt;sup&gt;1&lt;/sup&gt;, Mochammad Hatta&lt;sup&gt;2&lt;/sup&gt;, Syafri K.Arief&lt;sup&gt;3&lt;/sup&gt;, Tigor P. Simanjuntak&lt;sup&gt;4&lt;/sup&gt;, Bambang Suprayogi&lt;sup&gt;5&lt;/sup&gt; &lt;/strong&gt;&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;1&lt;/sup&gt;Department of Anesthesiology, Faculty of Medicine, Christian University of Indonesia, Jakarta, Indonesia&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;2&lt;/sup&gt;Molecular Biology and Immunology Laboratory, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;3&lt;/sup&gt;Department of Anesthesiology, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;4&lt;/sup&gt;Department of Obstetric and Gynecology, Faculty of Medicine, Christian University of Indonesia, Jakarta, Indonesia&lt;/p&gt;
&lt;p style=&quot;text-align: justify;&quot;&gt;&lt;sup&gt;5&lt;/sup&gt;Department of Otorhinolaryngology, Faculty of Medicine, Christian University of Indonesia, Jakarta, Indonesia&lt;/p&gt;</style></auth-address></record></records></xml>